Luo Wei, Wang Jinqiu, Chen Yan, Wang Yixu, Li Rui, Tang Jie, Geng Fang
Institute for Egg Science and Technology, School of Food and Biological Engineering, Chengdu University, No. 2025 Chengluo Avenue, Chengdu, 610106, China.
Engineering Research Center of Sichuan-Tibet Traditional Medicinal Plants, Chengdu University, No. 2025 Chengluo Avenue, Chengdu, 610106, China.
Curr Res Food Sci. 2022 Oct 9;5:1740-1749. doi: 10.1016/j.crfs.2022.09.029. eCollection 2022.
The survival mechanism of treated with high-intensity ultrasound (HIU) should be explored to further enhance the bactericidal efficacy of HIU. In this study, culturable was reduced by applying HIU. Electron microscope imaging revealed that HIU caused the disintegration of cell structure and leakage of intracellular substances. For the after the HIU treatment, key enzymes of the tricarboxylic acid [TCA] cycle were significantly downregulated, which led to a reduced ATP content (45.25%-75.00%), although ATPase activity was augmented by 33.82%-60.64% in the . Accordingly, surviving could have tolerated the stress of HIU by upregulating their environmental sensing (two-component system), chemotaxis (bacterial chemotaxis), substance uptake (ABC transporter), and ATP production (oxidative phosphorylation). Therefore, synergistically blocking the ATP production, signal transduction, or substance intake of offer promising potential strategies to improve the bactericidal effect of HIU in industrial food processing.
应探索高强度超声（HIU）处理后的存活机制，以进一步提高HIU的杀菌效果。在本研究中，应用HIU可减少可培养菌数量。电子显微镜成像显示，HIU导致细胞结构解体和细胞内物质泄漏。对于HIU处理后的细菌，三羧酸（TCA）循环的关键酶显著下调，这导致ATP含量降低（45.25%-75.00%），尽管细菌中的ATP酶活性增加了33.82%-60.64%。因此，存活的细菌可能通过上调其环境感知（双组分系统）、趋化性（细菌趋化）、物质摄取（ABC转运蛋白）和ATP产生（氧化磷酸化）来耐受HIU的压力。因此，协同阻断细菌的ATP产生、信号转导或物质摄取，为提高工业食品加工中HIU的杀菌效果提供了有前景的潜在策略。
