Quantitative proteomic analysis provides insight into the survival mechanism of typhimurium under high-intensity ultrasound treatment.

The survival mechanism of treated with high-intensity ultrasound (HIU) should be explored to further enhance the bactericidal efficacy of HIU. In this study, culturable was reduced by applying HIU. Electron microscope imaging revealed that HIU caused the disintegration of cell structure and leakage of intracellular substances. For the after the HIU treatment, key enzymes of the tricarboxylic acid [TCA] cycle were significantly downregulated, which led to a reduced ATP content (45.25%-75.00%), although ATPase activity was augmented by 33.82%-60.64% in the . Accordingly, surviving could have tolerated the stress of HIU by upregulating their environmental sensing (two-component system), chemotaxis (bacterial chemotaxis), substance uptake (ABC transporter), and ATP production (oxidative phosphorylation). Therefore, synergistically blocking the ATP production, signal transduction, or substance intake of offer promising potential strategies to improve the bactericidal effect of HIU in industrial food processing.