Łopusiewicz Łukasz, Bogusławska-Wąs Elżbieta, Drozłowska Emilia, Trocer Paulina, Dłubała Alicja, Mazurkiewicz-Zapałowicz Kinga, Bartkowiak Artur
Center of Bioimmobilisation and Innovative Packaging Materials, Faculty of Food Sciences and Fisheries, West Pomeranian University of Technology Szczecin, Janickiego 35, 71-270 Szczecin, Poland.
Department of Applied Microbiology and Human Nutrition Physiology, West Pomeranian University of Technology Szczecin, Papieża Pawła VI 3, 71-899 Szczecin, Poland.
Materials (Basel). 2021 Sep 15;14(18):5324. doi: 10.3390/ma14185324.
Agro-industrial by-products are promising source of biopolymers, including proteins and polysaccharides. This study was designed to evaluate the flaxseed oil cake extract (FOCE) as natural encapsulating material and carrier for probiotic GG (LGG). The powders were obtained using three spray drying inlet temperatures (110 °C, 140 °C, 170 °C), and reconstituted. The influence of temperature on water activity, morphology, chemical composition, flowability and cohesiveness of the powders was estimated. For all variants, the survival of bacteria during spray drying, and simulated passage through the gastrointestinal tract was evaluated. The preservation of LGG probiotic features such as cholesterol reduction, hydrophobicity and adhesion to mucin were examined. Results revealed that all physicochemical and functional characteristics of the powders were affected by the inlet temperature. This study demonstrated that FOCE is an appropriate matrix for spray drying (due to flaxseed proteins and polysaccharides) providing high survivability of bacteria (89.41-96.32%), that passed meaningfully through the simulated gastrointestinal tract (4.39-5.97 log reduction), largely maintaining their probiotic properties, being a promising environmentally-friendly carrier for probiotic LGG.
农产品加工副产品是生物聚合物（包括蛋白质和多糖）的有前景的来源。本研究旨在评估亚麻籽饼提取物（FOCE）作为益生菌GG（LGG）的天然包封材料和载体。使用三种喷雾干燥进口温度（110℃、140℃、170℃）获得粉末并进行复溶。评估了温度对粉末的水分活度、形态、化学成分、流动性和粘性的影响。对于所有变体，评估了喷雾干燥过程中细菌的存活率以及模拟胃肠道通过情况。检测了LGG益生菌特性（如胆固醇降低、疏水性和对粘蛋白的粘附性）的保留情况。结果表明，粉末的所有物理化学和功能特性均受进口温度影响。本研究表明，FOCE是喷雾干燥的合适基质（由于亚麻籽蛋白质和多糖），可提供高细菌存活率（89.41 - 96.32%），能有效通过模拟胃肠道（减少4.39 - 5.97个对数），并在很大程度上保持其益生菌特性，是益生菌LGG有前景的环保载体。
