Department of Food Science, Fundamental and Applied Research for Animal and Health, Faculty of Veterinary Medicine, University of Liège, Quartier Vallée 2, Avenue de Cureghem 10, 4000 Liège, Belgium.
Precision Livestock and Nutrition Unit, Gembloux Agro-Bio Tech, University of Liège, Passage des déportés 2, 5030 Gembloux, Belgium.
FEMS Microbiol Lett. 2020 Aug 1;367(16). doi: 10.1093/femsle/fnaa135.
The 'first 1000 days of life' determine the gut microbiota composition and can have long-term health consequences. In this study, the simulator of the human intestinal microbial ecosystem (SHIME®) model, which represents the main functional sections of the digestive tract, was chosen to study the microbiota of young children. The aim of this study was to reproduce the digestive process of toddlers and their specific colonic environment. The ascending, transverse and descending colons of SHIME® model were inoculated with feces from three donors aged between 1 and 2 years-old, in three separate runs. For each run, samples from colon vessels were collected at days 14, 21 and 28 after microbiota stabilization period. Short chain fatty acid concentrations determined by HPLC showed that microbiota obtained in SHIME® model shared characteristics between adults and infants. In addition, microbial diversity and bacterial populations determined by 16S rRNA amplicon sequencing were specific to each colon vessel. In conclusion, the SHIME® model developed in this study seemed well adapted to evaluate prebiotic and probiotic impact on the specific microbiota of toddlers, or medicine and endocrine disruptor metabolism. Moreover, this study is the first to highlight some biofilm development in in vitro gastrointestinal modelling systems.
“生命最初的 1000 天”决定了肠道微生物群落的组成，并可能对长期健康产生影响。在这项研究中，选择了模拟人类肠道微生物生态系统（SHIME®）的模型，该模型代表了消化道的主要功能部分，用于研究幼儿的微生物群。本研究的目的是复制幼儿的消化过程及其特定的结肠环境。将来自 3 名 1 至 2 岁供体的粪便接种到 SHIME®模型的升结肠、横结肠和降结肠中，在三个独立的运行中进行。对于每个运行，在微生物群稳定期后的第 14、21 和 28 天从结肠容器中采集样品。通过高效液相色谱法（HPLC）确定的短链脂肪酸浓度表明，在 SHIME®模型中获得的微生物群落具有成人和婴儿之间的特征。此外，通过 16S rRNA 扩增子测序确定的微生物多样性和细菌种群在每个结肠容器中都是特定的。总之，本研究中开发的 SHIME®模型似乎非常适合评估益生元和益生菌对幼儿特定微生物群的影响，或药物和内分泌干扰物的代谢。此外，这项研究首次强调了一些生物膜在体外胃肠道模拟系统中的发展。
