Plota Maria, Sazakli Eleni, Giormezis Nikolaos, Gkartziou Foteini, Kolonitsiou Fevronia, Leotsinidis Michalis, Antimisiaris Sophia G, Spiliopoulou Iris
Department of Microbiology, School of Medicine, University of Patras, 26504 Patras, Greece.
National Reference Centre for Staphylococci, School of Medicine, University of Patras, 26504 Patras, Greece.
Microorganisms. 2021 Aug 31;9(9):1853. doi: 10.3390/microorganisms9091853.
The purpose of the present study was to investigate anti-staphylococcal activity of daptomycin and bacteriophage K, alone or in combination, against biofilm-producers and non-producers and strains, under biofilm forming and cells' proliferation conditions. Daptomycin and bacteriophage K (ATCC 19685B1), in different concentrations, were tested against 10 and 10 , characterized by phenotypes and genotypes. The quantitative microtiter plate (crystal violet, CV), methylthiazoltetrazolium (MTT), and growth curve (GC) assays were performed. No statistically significant difference was found between species, whereas daptomycin alone performed better using medium and high concentrations of the drug and bacteriophage K was more active against strains with higher susceptibility, by CV and MTT assays. Best results were achieved using both agents combined in high concentrations. Bacteriophage K was effective within 3.8 and 2.4 h, depending on the concentration used, by the GC assay. Combination of daptomycin with bacteriophage K was more effective against staphylococci, depending on the concentrations used and strains' susceptibility. Further studies are needed to evaluate if this approach might be a choice for prevention or therapy of biofilm-associated infections.
本研究的目的是在生物膜形成和细胞增殖条件下，研究达托霉素和噬菌体K单独或联合使用时对生物膜产生菌和非产生菌以及菌株的抗葡萄球菌活性。以不同浓度的达托霉素和噬菌体K（ATCC 19685B1）对10株和10株通过表型和基因型进行表征的菌株进行测试。进行了定量微量滴定板（结晶紫，CV）、甲基噻唑基四氮唑（MTT）和生长曲线（GC）测定。在不同菌种之间未发现统计学上的显著差异，而通过CV和MTT测定，单独使用达托霉素时，中高浓度的药物效果更好，噬菌体K对敏感性较高的菌株更具活性。高浓度联合使用两种药物时取得了最佳效果。通过GC测定，噬菌体K在3.8小时和2.4小时内有效，具体取决于所使用的浓度。达托霉素与噬菌体K联合使用对葡萄球菌更有效，这取决于所使用的浓度和菌株的敏感性。需要进一步研究以评估这种方法是否可能成为预防或治疗生物膜相关感染的一种选择。
