Aranega-Bou Paz, Ellaby Nicholas, Ellington Matthew J, Moore Ginny
Biosafety, Air and Water Microbiology Group, National Infection Service, Public Health England, Manor Farm Rd, Porton Down, Salisbury SP4 0JG, UK.
Antimicrobial Resistance and Health Care Associated Infections, National infection Service, Public Health England, 61 Colindale Avenue, London NW9 5EQ, UK.
Microorganisms. 2021 Sep 3;9(9):1868. doi: 10.3390/microorganisms9091868.
Sink waste traps and drains are a reservoir for multi-drug resistant Gram-negative bacteria in the hospital environment. It has been suggested that these bacteria can migrate through hospital plumbing. Hospital waste traps were installed in a laboratory model system where sinks were connected through a common wastewater pipe. Enterobacterales populations were monitored using selective culture, MALDI-TOF identification and antibiotic resistance profiling before and after a wastewater backflow event. When transfer between sinks was suspected, isolates were compared using whole-genome sequencing. Immediately after the wastewater backflow, two KPC-producing were recovered from a waste trap in which Carbapenemase-producing Enterobacterales (CPE) had not been detected previously. The isolates belonged to ST501 and ST31 and were genetically indistinguishable to those colonising sinks elsewhere in the system. Following inter-sink transfer, KPC-producing ST501 successfully integrated into the microbiome of the recipient sink and was detected in the waste trap water at least five months after the backflow event. Seven weeks and three months after the backflow, other inter-sink transfers involving ST5295 and KPC-producing ST501 were also observed.
医院环境中，水槽废物存水弯和排水管道是耐多药革兰氏阴性菌的储存场所。有人提出，这些细菌可通过医院的管道系统迁移。在一个实验室模型系统中安装了医院废物存水弯，其中水槽通过一条共用废水管道相连。在废水回流事件前后，使用选择性培养、基质辅助激光解吸电离飞行时间质谱鉴定和抗生素耐药性分析对肠杆菌科菌群进行监测。当怀疑水槽之间存在细菌转移时，使用全基因组测序对分离株进行比较。废水回流后，立即从一个之前未检测到产碳青霉烯酶肠杆菌科细菌（CPE）的废物存水弯中分离出两种产KPC酶的菌株。这些分离株属于ST501和ST31，与系统中其他地方定殖于水槽的菌株在基因上无法区分。在水槽间转移后，产KPC酶的ST501成功整合到受体水槽的微生物群落中，并在回流事件至少五个月后在废物存水弯的水中被检测到。回流后七周和三个月，还观察到涉及ST5295和产KPC酶的ST501的其他水槽间转移。
