Krawczyk Agnieszka, Salamon Dominika, Kowalska-Duplaga Kinga, Bogiel Tomasz, Gosiewski Tomasz
Department of Molecular Medical Microbiology, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Krakow, Poland.
Department of Pediatrics, Gastroenterology and Nutrition, Faculty of Medicine, Jagiellonian University Medical College, 30-663 Krakow, Poland.
Pathogens. 2021 Sep 1;10(9):1119. doi: 10.3390/pathogens10091119.
The composition of bacteria is often altered in Crohn's disease (CD), but its connection to the disease is not fully understood. Gut archaea and fungi have recently been suggested to play a role as well. In our study, the presence and number of selected species of fungi and archaea in pediatric patients with CD and healthy controls were evaluated. Stool samples were collected from children with active CD ( = 54), non-active CD ( = 37) and control subjects ( = 33). The prevalence and the number of selected microorganisms were assessed by real-time PCR. The prevalence of was significantly increased in active CD compared to non-active CD and the control group ( = 0.011 and = 0.036, respectively). The number of spp. cells was significantly lower in patients with active CD compared to the control group, but in non-active CD, a significant increase was observed ( = 0.005 and = 0.020, respectively). There were no statistically significant differences in the colonization by archaea. The obtained results indicate possible correlations with the course of the CD; however, further studies of the entire archeobiome and the mycobiome are necessary in order to receive a complete picture.
克罗恩病（CD）患者的细菌组成常发生改变，但其与该疾病的关联尚未完全明确。最近有研究表明，肠道古菌和真菌也发挥了一定作用。在我们的研究中，对患有CD的儿科患者和健康对照中特定真菌和古菌种属的存在情况及数量进行了评估。从患有活动性CD的儿童（n = 54）、非活动性CD的儿童（n = 37）和对照受试者（n = 33）中收集粪便样本。通过实时PCR评估特定微生物的患病率和数量。与非活动性CD和对照组相比，活动性CD中[具体微生物名称]的患病率显著升高（分别为P = 0.011和P = 0.036）。与对照组相比，活动性CD患者中[具体微生物名称]细胞数量显著降低，但在非活动性CD中则观察到显著增加（分别为P = 0.005和P = 0.020）。古菌定植情况无统计学显著差异。所得结果表明可能与CD病程相关；然而，为了全面了解情况，有必要对整个古菌群落和真菌群落进行进一步研究。
