Center for Grain and Animal Health Research, Agricultural Research Service, United States Department of Agriculture, 1515 College Ave., Manhattan, KS 66502, USA.
National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, 3156 Rampart Road, Fort Collins, CO 80521, USA.
Viruses. 2021 Sep 14;13(9):1822. doi: 10.3390/v13091822.
Temperature plays a significant role in the vector competence, extrinsic incubation period, and intensity of infection of arboviruses within mosquito vectors. Most laboratory infection studies use static incubation temperatures that may not accurately reflect daily temperature ranges (DTR) to which mosquitoes are exposed. This could potentially compromise the application of results to real world scenarios. We evaluated the effect of fluctuating DTR versus static temperature treatments on the infection, dissemination, and transmission rates and viral titers of and mosquitoes for West Nile virus. Two DTR regimens were tested including an 11 and 15 °C range, both fluctuating around an average temperature of 28 °C. Overall, no significant differences were found between DTR and static treatments for infection, dissemination, or transmission rates for either species. However, significant treatment differences were identified for both and viral titers. These effects were species-specific and most prominent later in the infection. These results indicate that future studies on WNV infections in mosquitoes should consider employing realistic DTRs to reflect interactions most accurately between the virus, vector, and environment.
温度在蚊媒中虫媒病毒的媒介效能、外潜伏期和感染强度方面起着重要作用。大多数实验室感染研究使用静态孵育温度，而这些温度可能无法准确反映蚊子实际接触的日温度范围（DTR）。这可能会影响将研究结果应用于实际情况的能力。我们评估了波动 DTR 与静态温度处理对西尼罗河病毒感染、传播和传播率以及 和 蚊子的病毒滴度的影响。测试了两种 DTR 方案，包括 11 和 15°C 的范围，均围绕 28°C 的平均温度波动。总体而言，两种蚊种的感染、传播或传播率在 DTR 和静态处理之间没有发现显著差异。然而，两种蚊种的病毒滴度都存在显著的处理差异。这些影响具有物种特异性，在感染后期最为明显。这些结果表明，未来关于西尼罗河病毒在 蚊子中的感染研究应考虑采用现实的 DTR，以最准确地反映病毒、媒介和环境之间的相互作用。
