Dung Nguyen Thi, Trong Tran Duc, Vu Nguyen Thanh, Binh Nguyen Trong, Minh Tran Thi Le, Luan Le Quang
Biotechnology Center of Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam.
Faculty of Biologinal Sciences, Nong Lam University, Ho Chi Minh City 700000, Vietnam.
Nanomaterials (Basel). 2021 Sep 18;11(9):2439. doi: 10.3390/nano11092439.
Selenium nanoparticles (SeNPs) with diameters from 64.8 to 110.1 nm were successfully synthesized by γ-irradiation of solutions containing Se and water-soluble yeast -glucan. The size and size distribution of SeNPs were analyzed by dynamic light scattering (DLS). Analytical X-ray diffraction (XRD) pattern results confirmed the crystal structure of the Se nanoparticles and Fourier transform infrared (FTIR) spectroscopy revealed that -glucan could interact with SeNPs through steric (Se…O) linkages leading to a homogeneous and translucent solution state for 60 days without any precipitates. In vivo tests in cytoxan-induced immunosuppressed mice revealed that the daily supplementation of SeNPs/glucan at concentrations of 6 mg per kg body weight of tested mice significantly stimulated the generation of cellular immune factors (white blood cells, neutrophil, lymphocyte, B cells, CD4+ cells, CD34+ cells and natural killer cells) and humoral immune indexes (IgM, IgG, TNF-α, IFN-γ and IL-2) in peripheral blood, bone marrow and spleen of the immunosuppressed mice. The obtained results indicated that radiation-synthesized SeNPs/glucan may be a candidate for further evaluation as an agent for the prevention of immunosuppression in chemotherapy.
通过对含有硒和水溶性酵母β-葡聚糖的溶液进行γ辐射，成功合成了直径为64.8至110.1nm的硒纳米颗粒（SeNPs）。通过动态光散射（DLS）分析了SeNPs的尺寸和尺寸分布。X射线衍射（XRD）分析结果证实了硒纳米颗粒的晶体结构，傅里叶变换红外光谱（FTIR）显示β-葡聚糖可以通过空间（Se…O）键与SeNPs相互作用，从而形成均匀的半透明溶液状态，60天内无任何沉淀。对环磷酰胺诱导的免疫抑制小鼠进行的体内试验表明，以每千克受试小鼠体重6mg的浓度每日补充SeNPs/葡聚糖，可显著刺激免疫抑制小鼠外周血、骨髓和脾脏中细胞免疫因子（白细胞、中性粒细胞、淋巴细胞、B细胞、CD4+细胞、CD34+细胞和自然杀伤细胞）和体液免疫指标（IgM、IgG、TNF-α、IFN-γ和IL-2）的产生。所得结果表明，辐射合成的SeNPs/葡聚糖可能是一种有待进一步评估的预防化疗中免疫抑制的药物。
