College of Chemistry and Molecular Sciences, Wuhan University, Wuchang Luojiashan, Wuhan 430072, China.
College of Chemistry and Molecular Sciences, Wuhan University, Wuchang Luojiashan, Wuhan 430072, China.
Carbohydr Polym. 2015 Mar 6;117:434-442. doi: 10.1016/j.carbpol.2014.09.088. Epub 2014 Oct 12.
We report on a green procedure for the stabilization of selenium nanoparticles (SeNPs) by a naturally occurring β-glucan with triple helical conformation known as Lentinan (t-LNT) in water after denaturing into single chains (s-LNT) at 140 °C. The results demonstrated that the s-LNT can interact with SeNPs through Se-O-H interaction. Transmission electron microscopy (TEM), energy dispersive X-ray (EDX) spectra, UV/vis, X-ray diffraction (XRD) and dynamic light scattering (DLS) showed that s-LNT coated SeNPs to form a stable nano-composite Se/s-LNT, leading to good dispersion of SeNPs. Especially, the as-prepared Se/s-LNT composite in the solution could remain homogeneous and translucent for 30 days without any precipitates. Different size distribution of SeNPs was prepared by simply controlling the concentrations of selenite sodium and the corresponding reducing agent ascorbic acid. The size effect of SeNPs on anti-tumor activity was revealed that the SeNPs with more evenly particle size distribution show the higher anticancer activity.
我们报道了一种绿色的方法，即在 140°C 下将具有三重螺旋构象的天然β-葡聚糖（Lentinan，t-LNT）变性为单链（s-LNT），然后用其稳定硒纳米粒子（SeNPs）。结果表明，s-LNT 可以通过 Se-O-H 相互作用与 SeNPs 相互作用。透射电子显微镜（TEM）、能谱（EDX）谱、紫外-可见（UV/vis）、X 射线衍射（XRD）和动态光散射（DLS）表明，s-LNT 包覆 SeNPs 形成了稳定的纳米复合材料 Se/s-LNT，导致 SeNPs 良好分散。特别是，在溶液中制备的 Se/s-LNT 复合材料在 30 天内仍然保持均匀和半透明，没有任何沉淀。通过简单地控制亚硒酸钠的浓度和相应的还原剂抗坏血酸，可以制备出不同粒径分布的 SeNPs。SeNPs 的尺寸效应对抗肿瘤活性的影响表明，粒径分布更均匀的 SeNPs 具有更高的抗癌活性。
