Tezuka Daisuke, Matsuura Hideyuki, Saburi Wataru, Mori Haruhide, Imai Ryozo
Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba 305-8604, Japan.
Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.
Plants (Basel). 2021 Sep 10;10(9):1875. doi: 10.3390/plants10091875.
Salicylic acid (SA) is a phytohormone that regulates a variety of physiological and developmental processes, including disease resistance. SA is a key signaling component in the immune response of many plant species. However, the mechanism underlying SA-mediated immunity is obscure in rice (). Prior analysis revealed a correlation between basal SA level and blast resistance in a range of rice varieties. This suggested that resistance might be improved by increasing basal SA level. Here, we identified a novel UDP-glucosyltransferase gene, , which is expressed ubiquitously throughout plant development. Mutants of generated by genome editing accumulated high levels of SA under non-stressed conditions, indicating that is a key enzyme for SA homeostasis in rice. Microarray analysis revealed that the mutants constitutively overexpressed a set of pathogenesis-related (PR) genes. An inoculation assay demonstrated that these mutants had increased resistance against rice blast, but they also exhibited stunted growth phenotypes. To our knowledge, this is the first report of a rice mutant displaying SA overaccumulation.
水杨酸（SA）是一种植物激素，可调节多种生理和发育过程，包括抗病性。SA是许多植物物种免疫反应中的关键信号成分。然而，SA介导的免疫在水稻中的潜在机制尚不清楚。先前的分析揭示了一系列水稻品种中基础SA水平与稻瘟病抗性之间的相关性。这表明通过提高基础SA水平可能会增强抗性。在此，我们鉴定了一个新的UDP-葡萄糖基转移酶基因，该基因在植物发育过程中普遍表达。通过基因组编辑产生的该基因的突变体在非胁迫条件下积累了高水平的SA，表明该基因是水稻中SA稳态的关键酶。微阵列分析显示，该基因突变体组成型过表达一组病程相关（PR）基因。接种试验表明，这些突变体对稻瘟病的抗性增强，但它们也表现出生长发育迟缓的表型。据我们所知，这是关于水稻突变体显示SA过度积累的首次报道。
