College of Biological Sciences and Technology, Taiyuan Normal University, Taiyuan, 030619, China.
MARA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
BMC Plant Biol. 2024 Apr 6;24(1):249. doi: 10.1186/s12870-024-04926-8.
Tartary buckwheat (Fagopyrum tataricum) belongs to Polygonaceae family and has attracted increasing attention owing to its high nutritional value. UDP-glycosyltransferases (UGTs) glycosylate a variety of plant secondary metabolites to control many metabolic processes during plant growth and development. However, there have been no systematic reports of UGT superfamily in F. tataricum.
We identified 173 FtUGTs in F. tataricum based on their conserved UDPGT domain. Phylogenetic analysis of FtUGTs with 73 Arabidopsis UGTs clustered them into 21 families. FtUGTs from the same family usually had similar gene structure and motif compositions. Most of FtUGTs did not contain introns or had only one intron. Tandem repeats contributed more to FtUGTs amplification than segmental duplications. Expression analysis indicates that FtUGTs are widely expressed in various tissues and likely play important roles in plant growth and development. The gene expression analysis response to different abiotic stresses showed that some FtUGTs were involved in response to drought and cadmium stress. Our study provides useful information on the UGTs in F. tataricum, and will facilitate their further study to better understand their function.
Our results provide a theoretical basis for further exploration of the functional characteristics of FtUGTs and for understanding the growth, development, and metabolic model in F. tataricum.
苦荞（Fagopyrum tataricum）属于蓼科，由于其营养价值高，近年来受到越来越多的关注。UDP-糖基转移酶（UGTs）将各种植物次生代谢物糖基化，以控制植物生长和发育过程中的许多代谢过程。然而，在 F. tataricum 中，UGT 超家族还没有系统的报道。
我们根据保守的 UDPGT 结构域在 F. tataricum 中鉴定了 173 个 FtUGTs。与 73 个拟南芥 UGTs 的 FtUGTs 系统发育分析将它们聚类为 21 个家族。来自同一家族的 FtUGTs 通常具有相似的基因结构和基序组成。大多数 FtUGTs 不含内含子或只有一个内含子。串联重复对 FtUGTs 的扩增贡献大于片段重复。表达分析表明，FtUGTs 在各种组织中广泛表达，可能在植物生长和发育中发挥重要作用。对不同非生物胁迫的基因表达分析表明，一些 FtUGTs 参与了对干旱和镉胁迫的响应。我们的研究为 F. tataricum 中的 UGTs 提供了有用的信息，并将有助于进一步研究它们的功能。
我们的结果为进一步探索 FtUGTs 的功能特征以及理解 F. tataricum 的生长、发育和代谢模型提供了理论基础。
