华东地区老化油泥污染土壤的细菌群落结构通过 Illumina 高通量测序揭示。

Bacterial community structure of aged oil sludge contaminated soil revealed by illumina high-throughput sequencing in East China.

机构信息

College of Environment and Safety Engineering, Qingdao University of Science and Technology, Shandong Province, Qingdao, 266042, People's Republic of China.

Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, College of Chemistry and Molecular Engineering, MOE, Qingdao University of Science and Technology, Shandong Province, Qingdao, 266042, People's Republic of China.

出版信息

World J Microbiol Biotechnol. 2021 Sep 28;37(11):183. doi: 10.1007/s11274-021-03059-6.

DOI:10.1007/s11274-021-03059-6

PMID:34580778

Abstract

Screening of the dominant or core oil resistant bacteria in Aged Oil Sludge (AOS) contaminated soil in Daqing and Shengli oilfields (DQ and SL) in China was investigated through High-Throughput Sequencing method. Enhanced total organic carbon (TOC, 12.53 to 28.35 g/kg in DQ and 3.07 to 4.97 g/kg in SL) and total petroleum hydrocarbons (TPHs, 21 to 2837 mg/mg in DQ and 13 to 1558 mg/kg in SL) were observed. The internal transcribed spacer (ITS) sequencing by Illumine Miseq platform at each taxonomic level revealed the notable toxicological effect of AOS on the diversity and community structure of bacteria. In this study, sequence analyses showed 77-89% and 92-98% reduction of Firmicutes at phylum level in DQ and SL respectively after treated with AOS. Enhanced universal gene location was observed in Proteobacteria, Actinobacteria, Gemmatimonadetes and Bacteroidetes in DQ and SL. The universal dominant family in the two oilfields was anaerolineaceae. At the genus level, Algiphilus in DQ and Pseudomonas in SL were the majority respectively. In total, 3 negligible genera (Perlucidibaca, Alcanivorax and Algiphilus) in DQ and 13 negligible genera (Salinisphaera, Microbulbifer and Idiomarina, et al.,) in SL were significantly enriched after oil treatment indicating their possible role in the attenuation of petroleum hydrocarbons.

摘要

通过高通量测序方法研究了中国大庆油田（DQ）和胜利油田（SL）老化油污泥（AOS）污染土壤中优势或核心耐油菌的筛选。观察到增强的总有机碳（TOC，DQ 为 12.53 至 28.35 g/kg，SL 为 3.07 至 4.97 g/kg）和总石油烃（TPHs，DQ 为 21 至 2837 mg/mg，SL 为 13 至 1558 mg/kg）。Illumine Miseq 平台的内部转录间隔区（ITS）测序在每个分类水平上揭示了 AOS 对细菌多样性和群落结构的显著毒性作用。在这项研究中，序列分析表明，DQ 和 SL 经 AOS 处理后厚壁菌门的丰度分别降低了 77-89%和 92-98%。在 DQ 和 SL 中，变形菌门、放线菌门、芽单胞菌门和拟杆菌门的增强普遍基因定位观察到。两个油田普遍存在的优势家族是厌氧杆菌科。在属水平上，DQ 中的 Algiphilus 和 SL 中的 Pseudomonas 分别是优势菌。总的来说，DQ 中有 3 个可忽略的属（Perlucidibaca、Alcanivorax 和 Algiphilus），SL 中有 13 个可忽略的属（Salinisphaera、Microbulbifer 和 Idiomarina 等），在油处理后明显富集，表明它们可能在石油烃的衰减中发挥作用。