Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China; University of the Chinese Academy of Sciences, Beijing, 100049, China.
Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
Chemosphere. 2023 May;322:138225. doi: 10.1016/j.chemosphere.2023.138225. Epub 2023 Feb 22.
Surfactant-enhanced bioremediation (SEBR) is frequently employed to clean up soil polluted with petroleum hydrocarbons, but few studies have focused on how surfactants affect microbial communities and different fractions of petroleum hydrocarbons, particularly in the field. Here, the surfactants sodium dodecyl benzene sulfonate (SDBS), alpha olefin sulfonate (AOS), Triton X-100 (TX-100), Tween80, and rhamnolipid were combined with the oil-degrading bacterium Pseudomonas sp. SB to remediate oil-contaminated soil in the laboratory. AOS gave the highest removal efficiency (65.1%) of total petroleum hydrocarbons (TPHs). Therefore, AOS was used in a field experiment with Pseudomonas sp. SB and the removal efficiency of TPHs and long-chain hydrocarbons C21-C40 reached 57.4 and 53.0%, respectively, significantly higher than the other treatments. During bioremediation the addition of Pseudomonas sp. SB significantly stimulated the growth of bacterial genera such as Alcanivorax, Luteimonas, Parvibaculum, Stenotrophomonas, and Pseudomonas and AOS further stimulated the growth of Sphingobacterium, Pseudomonas and Alcanivorax. This study validates the feasibility of surfactant-enhanced bioremediation in the field and partly reveals the mechanism of surfactant-enhanced bioremediation from the perspective of changes in different fractions of petroleum and microbial community dynamics.
表面活性剂强化生物修复（SEBR）常用于清除受石油烃污染的土壤，但很少有研究关注表面活性剂如何影响微生物群落和不同石油烃馏分，尤其是在野外。本研究在实验室中用十二烷基苯磺酸钠（SDBS）、α-烯烃磺酸盐（AOS）、Triton X-100（TX-100）、吐温 80 和鼠李糖脂与石油降解菌假单胞菌 SB 联合修复石油污染土壤。AOS 对总石油烃（TPH）的去除效率最高（65.1%）。因此，AOS 与假单胞菌 SB 一起用于野外试验，TPH 和长链烃 C21-C40 的去除效率分别达到 57.4%和 53.0%，显著高于其他处理。在生物修复过程中，添加假单胞菌 SB 显著刺激了 Alcanivorax、Luteimonas、Parvibaculum、Stenotrophomonas 和 Pseudomonas 等细菌属的生长，AOS 进一步刺激了 Sphingobacterium、Pseudomonas 和 Alcanivorax 的生长。本研究验证了表面活性剂强化生物修复在野外的可行性，并从石油不同馏分和微生物群落动态变化的角度部分揭示了表面活性剂强化生物修复的机制。
