Shi Jing-Yi, Cai Wen-Jun, Lin Wen-Dong, Zhang Shuo, Luo Rong
School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China.
Zhongguo Zhong Yao Za Zhi. 2021 Sep;46(17):4446-4455. doi: 10.19540/j.cnki.cjcmm.20210618.203.
Twenty batches of Aurantii Fructus Immaturus(AFI) were collected, with their peel and pulp taken as research objects. Ultra-high performance liquid chromatography(UPLC) fingerprints of peel and pulp of AFI were established with 17 common peaks in peel and 10 in pulp. Six kinds of flavonoids were identified, i.e., narirutin, naringin, rhoifolin, hesperidin, neohesperidin and nobiletin. The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine was employed for similarity analysis, which showed that the chromatographic peaks of peel and pulp were basically similar to their respective reference fingerprints, with all similarities greater than 0.90. The similarity between peel and pulp of the same batch of AFI ranged from 0.850 to 0.983. Cluster analysis(CA), principal component analysis(PCA), and orthogonal partial least squares discriminant analysis(OPLS-DA) were conducted on the common peaks of peel and pulp of AFI with SPSS 17.0 and SIMCA 14.1. Combined with the reference fingerprints, these analyses revealed 12 differential components regarding peel and pulp. Further, the content of the 6 flavonoids and synephrine was determined. The proposed method integrating UPLC fingerprint and multicomponent quantitative analysis is applicable to the quality evaluation of AFI. The results provide a certain basis for the scientific connotation about the appearance characteristic of AFI.
收集了20批枳实，以其果皮和果肉为研究对象。建立了枳实果皮和果肉的超高效液相色谱（UPLC）指纹图谱，果皮中有17个共有峰，果肉中有10个共有峰。鉴定出6种黄酮类化合物，即橙皮芸香苷、柚皮苷、异橙皮苷、橙皮苷、新橙皮苷和川陈皮素。采用《中药色谱指纹图谱相似度评价系统》进行相似度分析，结果表明，果皮和果肉的色谱峰与其各自的对照指纹图谱基本相似，相似度均大于0.90。同一批枳实果皮与果肉的相似度范围为0.850～0.983。用SPSS 17.0和SIMCA 14.1软件对枳实果皮和果肉的共有峰进行聚类分析（CA）、主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS-DA）。结合对照指纹图谱，这些分析揭示了果皮和果肉的12个差异成分。此外，还测定了6种黄酮类化合物和辛弗林的含量。所提出的结合UPLC指纹图谱和多成分定量分析的方法适用于枳实的质量评价。研究结果为枳实外观特征的科学内涵提供了一定依据。
