Liu Zi-Xuan, Fu Xue-Sen, Wang Ling, Liu Xiang-Dan, Zhou Ri-Bao
School of Pharmacy, Hunan University of Chinese Medicine Changsha 410208, China Key Research Laboratory of Germplasm Resources and Standardized Planting of Genuine Regional Medicinal Materials Produced in Hunan Province Changsha 410208, China Key Laboratory of Modern Research of Traditional Chinese Medicine, Education Department of Hunan Province Changsha 410208, China.
Zhongguo Zhong Yao Za Zhi. 2023 Jan;48(1):265-272. doi: 10.19540/j.cnki.cjcmm.20221015.101.
The present study explored the consistency of the content proportions of active components of Aurantii Fructus and analyzed the influencing factors based on three-dimensional multi-component analysis. A total of 839 Aurantii Fructus samples in 65 research articles were analyzed using the three-dimensional multi-component analysis mode. The content data of flavonoid components(naringin, hesperidin, neohesperidin, narirutin, and nobiletin), coumarin components(meranzin and gluconolactone), and alkaloid(synephrine) in 386 samples which met the criteria of 2020 edition of the Chinese Pharmacopoeia were extracted and adjusted to percentages, and the content ratios between components were calculated. The influencing factors of Aurantii Fructus quality were analyzed. The results showed content ratios of components as follows: neohesperidin∶naringin in the range of 0.4-1.2; narirutin∶naringin in the range of 0.02-0.16; hesperidin∶naringin in the range of 0.01-0.3; nobiletin∶naringin in the range of 0.000 588 3-0.069 68; synephrine∶naringin in the range of 0.02-0.042; gluconolactone∶naringin in the range of 0.001-0.01; meranzin∶naringin in the range of 0.000 4-0.035. The quality of Aurantii Fructus was closely related to the origin, variety, harvesting time, and processing method of medicinal materials. Harvesting time had a greater impact on the quality of Aurantii Fructus, and the origin and variety had a certain impact on the quality of Aurantii Fructus. The findings of this study indicated that the ratios between flavonoid components, flavonoids and coumarin components, and flavonoids and alkaloids fluctuated. The production base should optimize the varieties, harvesting period, and processing methods of Aurantii Fructus to provide a scientific basis for the production of high-quality Aurantii Fructus.
本研究探讨了枳壳活性成分含量比例的一致性，并基于三维多成分分析对影响因素进行了分析。采用三维多成分分析模式，对65篇研究论文中的839个枳壳样本进行了分析。提取了386个符合2020年版《中国药典》标准的样本中黄酮类成分（柚皮苷、橙皮苷、新橙皮苷、柚皮芸香苷和诺米林）、香豆素类成分（枸橼内酯和葡萄糖酸内酯）和生物碱（辛弗林）的含量数据，并将其调整为百分比，计算各成分之间的含量比值。分析了枳壳质量的影响因素。结果显示各成分的含量比值如下：新橙皮苷∶柚皮苷为0.4 - 1.2；柚皮芸香苷∶柚皮苷为0.02 - 0.16；橙皮苷∶柚皮苷为0.01 - 0.3；诺米林∶柚皮苷为0.000 588 3 - 0.069 68；辛弗林∶柚皮苷为0.02 - 0.042；葡萄糖酸内酯∶柚皮苷为0.001 - 0.01；枸橼内酯∶柚皮苷为0.000 4 - 0.035。枳壳质量与药材的产地、品种、采收时间和加工方法密切相关。采收时间对枳壳质量影响较大，产地和品种对枳壳质量有一定影响。本研究结果表明，黄酮类成分之间、黄酮类与香豆素类成分之间以及黄酮类与生物碱类之间的比值存在波动。生产基地应优化枳壳的品种、采收期和加工方法，为高品质枳壳的生产提供科学依据。
