牛卵泡液中抑制素两个亚基编码cDNA物种的克隆与序列分析。
Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid.
作者信息
Forage R G, Ring J M, Brown R W, McInerney B V, Cobon G S, Gregson R P, Robertson D M, Morgan F J, Hearn M T, Findlay J K
出版信息
Proc Natl Acad Sci U S A. 1986 May;83(10):3091-5. doi: 10.1073/pnas.83.10.3091.
Abstract
The primary amino acid structures of the 43-kDa (A) and 15-kDa (B) subunits of the 58-kDa form of the hormone inhibin have been elucidated by cloning and analysis of cDNA species derived from bovine granulosa cell mRNA. The A subunit (Mr = 32,298) is a protein of 300 amino acids with two potential N-glycosylation sites and two potential proteolytic processing sites and has a pre-pro region of 60 amino acids. The mature B subunit (Mr = 12,977) is a protein of 116 amino acids synthesized from a separate mRNA. These data establish that a 31-kDa form of inhibin also isolated from bovine follicular fluid, with subunits of 20 kDa (Ac) and 15 kDa (B), is derived from the 58-kDa form by proteolytic processing of the A subunit.
摘要
通过对源自牛颗粒细胞mRNA的cDNA进行克隆和分析，已阐明了58 kDa形式的激素抑制素的43 kDa（A）和15 kDa（B）亚基的一级氨基酸结构。A亚基（Mr = 32,298）是一种由300个氨基酸组成的蛋白质，具有两个潜在的N-糖基化位点和两个潜在的蛋白水解加工位点，并且有一个由60个氨基酸组成的前原区域。成熟的B亚基（Mr = 12,977）是一种由单独的mRNA合成的116个氨基酸的蛋白质。这些数据表明，同样从牛卵泡液中分离出的、具有20 kDa（Ac）和15 kDa（B）亚基的31 kDa形式的抑制素，是通过A亚基的蛋白水解加工从58 kDa形式衍生而来的。
相似文献
1
Cloning and sequence analysis of cDNA species coding for the two subunits of inhibin from bovine follicular fluid.牛卵泡液中抑制素两个亚基编码cDNA物种的克隆与序列分析。
Proc Natl Acad Sci U S A. 1986 May;83(10):3091-5. doi: 10.1073/pnas.83.10.3091.
2
Purification and characterization of high molecular weight forms of inhibin from bovine follicular fluid.牛卵泡液中高分子量抑制素形式的纯化与特性分析
Endocrinology. 1992 Feb;130(2):789-96. doi: 10.1210/endo.130.2.1733725.
3
[cDNA cloning and mRNA expression pattern in follicles of the mature inhibin alpha subunit from Xianju chicken].[仙居鸡成熟抑制素α亚基的cDNA克隆及卵泡中的mRNA表达模式]
Yi Chuan Xue Bao. 2001 Aug;28(8):707-15.
4
Inhibin A-subunit cDNAs from porcine ovary and human placenta.来自猪卵巢和人胎盘的抑制素A亚基互补DNA。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5849-53. doi: 10.1073/pnas.83.16.5849.
5
Molecular cloning and expression analysis of the complementary deoxyribonucleic acid for chicken inhibin/activin beta(B) subunit.鸡抑制素/激活素β（B）亚基互补脱氧核糖核酸的分子克隆与表达分析
Biol Reprod. 2000 May;62(5):1128-34. doi: 10.1095/biolreprod62.5.1128.
6
Complementary DNA sequences of ovarian follicular fluid inhibin show precursor structure and homology with transforming growth factor-beta.卵巢卵泡液抑制素的互补DNA序列显示出前体结构，并与转化生长因子-β具有同源性。
Nature. 1985;318(6047):659-63. doi: 10.1038/318659a0.
7
Complementary deoxyribonucleic acid cloning and sequence analysis of the alpha-subunit of inhibin from chicken ovarian granulosa cells.鸡卵巢颗粒细胞抑制素α亚基的互补脱氧核糖核酸克隆及序列分析
Biol Reprod. 1993 Sep;49(3):453-8. doi: 10.1095/biolreprod49.3.453.
8
Inhibin alpha-subunit monomer is present in bovine follicular fluid.抑制素α亚基单体存在于牛卵泡液中。
Biochem Biophys Res Commun. 1989 Mar 31;159(3):1323-9. doi: 10.1016/0006-291x(89)92255-9.
9
Rat inhibin: molecular cloning of alpha- and beta-subunit complementary deoxyribonucleic acids and expression in the ovary.大鼠抑制素：α和β亚基互补脱氧核糖核酸的分子克隆及在卵巢中的表达。
Mol Endocrinol. 1987 Aug;1(8):561-8. doi: 10.1210/mend-1-8-561.
10
Molecular cloning and expression of turkey inhibin-alpha and -betaA subunits.火鸡抑制素α亚基和βA亚基的分子克隆与表达
Poult Sci. 2001 Dec;80(12):1690-4. doi: 10.1093/ps/80.12.1690.
引用本文的文献
1
Insights into in vivo follicle formation: a review of in vitro systems.体内卵泡形成的研究进展：体外体系的综述。
Histochem Cell Biol. 2022 Mar;157(3):333-345. doi: 10.1007/s00418-021-02058-w. Epub 2021 Nov 30.
2
Cloning expression and immunogenicity analysis of inhibin gene in Ye Mule Aries sheep.野穆尔白羊抑制素基因的克隆表达及免疫原性分析
PeerJ. 2019 Sep 25;7:e7761. doi: 10.7717/peerj.7761. eCollection 2019.
3
Inhibin at 90: from discovery to clinical application, a historical review.抑制素90年：从发现到临床应用，历史回顾
Endocr Rev. 2014 Oct;35(5):747-94. doi: 10.1210/er.2014-1003. Epub 2014 Jul 22.
4
Characterization of the mechanism of inhibin α-subunit gene in mouse anterior pituitary cells by RNA interference.RNA干扰对小鼠垂体前叶细胞中抑制素α亚基基因作用机制的研究
PLoS One. 2013 Oct 3;8(10):e74596. doi: 10.1371/journal.pone.0074596. eCollection 2013.
5
Expression of inhibin-alpha is regulated synergistically by Wilms' tumor gene 1 (Wt1) and steroidogenic factor-1 (Sf1) in sertoli cells.抑制素-α的表达受睾丸细胞瘤基因 1（Wt1）和类固醇生成因子-1（Sf1）在支持细胞中的协同调节。
PLoS One. 2013;8(1):e53140. doi: 10.1371/journal.pone.0053140. Epub 2013 Jan 11.
6
Physical mapping of inhibin beta-A in domestic cattle.家牛中抑制素β - A的物理图谱
Mamm Genome. 1993;4(6):328-32. doi: 10.1007/BF00357092.
7
Complete amino acid sequence of BSP-A3 from bovine seminal plasma. Homology to PDC-109 and to the collagen-binding domain of fibronectin.牛精浆中BSP-A3的完整氨基酸序列。与PDC-109以及纤连蛋白的胶原结合结构域的同源性。
Biochem J. 1987 Apr 1;243(1):195-203. doi: 10.1042/bj2430195.
8
Heterodimers and homodimers of inhibin subunits have different paracrine action in the modulation of luteinizing hormone-stimulated androgen biosynthesis.抑制素亚基的异二聚体和同二聚体在调节促黄体生成素刺激的雄激素生物合成中具有不同的旁分泌作用。
Proc Natl Acad Sci U S A. 1987 Jul;84(14):5082-6. doi: 10.1073/pnas.84.14.5082.
9
Erythroid differentiation factor is encoded by the same mRNA as that of the inhibin beta A chain.红细胞分化因子由与抑制素βA链相同的信使核糖核酸编码。
Proc Natl Acad Sci U S A. 1988 Apr;85(8):2434-8. doi: 10.1073/pnas.85.8.2434.
10
Relative roles of follicle-stimulating hormone and luteinizing hormone in the control of inhibin secretion in normal men.促卵泡激素和促黄体生成素在正常男性抑制素分泌调控中的相对作用。
J Clin Invest. 1988 Sep;82(3):880-4. doi: 10.1172/JCI113693.
本文引用的文献
1
DUAL ENDOCRINE ACTIVITY OF THE TESTES.睾丸的双重内分泌活动
Science. 1932 Jul 1;76(1957):19-20. doi: 10.1126/science.76.1957.19.
2
Transformation of E. coli using homopolymer-linked plasmid chimeras.使用同聚物连接的质粒嵌合体转化大肠杆菌。
Biochim Biophys Acta. 1981 Sep 28;655(2):243-50. doi: 10.1016/0005-2787(81)90014-9.
3
Plasmid screening at high colony density.高菌落密度下的质粒筛选
Gene. 1980 Jun;10(1):63-7. doi: 10.1016/0378-1119(80)90144-4.
4
Processing mechanisms in the biosynthesis of proteins.蛋白质生物合成中的加工机制。
Ann N Y Acad Sci. 1980;343:1-16. doi: 10.1111/j.1749-6632.1980.tb47238.x.
5
Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis.从聚丙烯酰胺凝胶中分离微克量蛋白质用于氨基酸序列分析。
Methods Enzymol. 1983;91:227-36. doi: 10.1016/s0076-6879(83)91019-4.
6
Evidence for the prostatic origin of immunoreactive inhibin-like material in human seminal plasma.人精浆中免疫反应性抑制素样物质来源于前列腺的证据。
Int J Androl. 1984 Oct;7(5):389-97. doi: 10.1111/j.1365-2605.1984.tb00796.x.
7
Complete amino acid sequence of human seminal plasma beta-inhibin. Prediction of post Gln-Arg cleavage as a maturation site.人精浆β-抑制素的完整氨基酸序列。预测Gln-Arg切割后作为成熟位点。
FEBS Lett. 1984 Oct 1;175(2):349-55. doi: 10.1016/0014-5793(84)80766-8.
8
Analysis of an inhibin preparation reveals apparent identity between a peptide with inhibin-like activity and a sperm-coating antigen.对一种抑制素制剂的分析显示，一种具有抑制素样活性的肽与一种精子包被抗原有明显的一致性。
FEBS Lett. 1984 Oct 15;176(1):21-6. doi: 10.1016/0014-5793(84)80904-7.
9
Compilation of published signal sequences.已发表信号序列的汇编。
Nucleic Acids Res. 1984 Jul 11;12(13):5145-64. doi: 10.1093/nar/12.13.5145.
10
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
Zotero 插件