家牛中抑制素β - A的物理图谱
Physical mapping of inhibin beta-A in domestic cattle.
作者信息
Neibergs H L, Gallagher D S, Georges M, Sargeant L S, Dietz A B, Womack J E
机构信息
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843.
出版信息
Mamm Genome. 1993;4(6):328-32. doi: 10.1007/BF00357092.
The gene for the beta-A subunit of inhibin (INHBA) was assigned to bovine syntenic group U13 by bovine x rodent hybrid somatic cells and the polymerase chain reaction (PCR). A 482-bp PCR fragment was used to clone a 37-kb cosmid. This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). This is the first assignment of a U13 marker to a bovine chromosome. A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid.
通过牛与啮齿动物的杂种体细胞和聚合酶链反应（PCR），将抑制素β - A亚基（INHBA）基因定位到牛的同线群U13。用一个482bp的PCR片段克隆了一个37kb的黏粒。通过荧光原位杂交（FISH）将该黏粒定位到牛的4号染色体（BTA 4）上。这是首次将U13标记定位到牛染色体上。在INHBA黏粒中检测到PstI限制性片段长度多态性（RFLP）。
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