Department of Systems and Computer Engineering, Carleton University, Ottawa, Ontario, Canada K1S 5B6.
Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, Ontario, Canada K1A 0C6.
J Proteome Res. 2021 Nov 5;20(11):4925-4947. doi: 10.1021/acs.jproteome.1c00138. Epub 2021 Sep 28.
The soybean crop, (L.) Merr., is consumed by humans, , worldwide. While the respective bodies of literature and - data for each of these organisms are extensive, comparatively few studies investigate the molecular biological processes occurring between the two. We are interested in elucidating the network of protein-protein interactions (PPIs) involved in human-soybean allergies. To this end, we leverage state-of-the-art sequence-based PPI predictors amenable to predicting the enormous comprehensive interactome between human and soybean. A network-based analytical approach is proposed, leveraging to identify candidate allergens and proteins involved in the allergy response. Interestingly, the predicted interactome can be explored from two complementary perspectives: and . A total of eight proteins (six specific to the human proteome and two to the soy proteome) have been identified and supported by the literature to be involved in human health, specifically related to immunological and neurological pathways. This study, beyond generating the most comprehensive human-soybean interactome to date, elucidated a soybean seed interactome and identified several proteins putatively consequential to human health.
大豆作物（L.）是一种被全球人类消费的农作物。虽然这两个生物体各自的文献和数据都非常丰富，但相对较少的研究调查了它们之间发生的分子生物学过程。我们有兴趣阐明涉及人类与大豆过敏反应的蛋白质-蛋白质相互作用（PPIs）网络。为此，我们利用了最新的基于序列的 PPI 预测器，这些预测器可用于预测人类和大豆之间庞大的综合相互作用组。提出了一种基于网络的分析方法，利用它来识别候选过敏原和参与过敏反应的蛋白质。有趣的是，预测的相互作用组可以从两个互补的角度进行探索：人类和大豆。总共鉴定出了 8 种蛋白质（6 种特定于人类蛋白质组，2 种特定于大豆蛋白质组），并得到文献支持，这些蛋白质与人类健康有关，特别是与免疫和神经途径有关。这项研究不仅生成了迄今为止最全面的人类-大豆相互作用组，还阐明了大豆种子相互作用组，并确定了几种可能对人类健康有影响的蛋白质。
