The contribution of phenolics to the anti-inflammatory potential of the extract from Bolivian coriander (Porophyllum ruderale subsp. ruderale).

Porophyllum ruderale subsp. ruderale is a food product used for seasoning in Central and Southern America. The present research aimed to investigate the chemical composition of an extract prepared from aerial parts of P. ruderale using UHPLC-DAD-MS/MS, to isolate and identify major natural products present in the extract, and to furtherly investigate their anti-inflammatory activity in vitro. Twenty-five compounds were detected and characterized using UV-Vis and MS data. All characterized compounds were quantified. Ten major phenolics were isolated and identified by NMR. One previously undescribed natural product was isolated and established as 1-O-(4-hydroxy-3,5-dimethoxy)benzoyl-6-O-galloyl-β-d-glucose (12). Anti-inflammatory activity was evaluated based on the influence of the extract and isolated compounds on the TLR4-dependent secretion of IL-8 and TNF-α by human primary neutrophils in vitro. Phenolic acids, and caffeic acid derivatives in particular, contributed to the extract's bioactivity.