Department of Pharmacognosy and Molecular Basis of Phytotherapy, Centre for Preclinical Studies, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland; Microbiota Lab, Centre for Preclinical Studies, Medical University of Warsaw, ul. Banacha 1b, 02-097 Warsaw, Poland.
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Centre for Preclinical Studies, Medical University of Warsaw, ul. Banacha 1, 02-097 Warsaw, Poland.
Food Chem. 2022 Mar 1;371:131116. doi: 10.1016/j.foodchem.2021.131116. Epub 2021 Sep 14.
Porophyllum ruderale subsp. ruderale is a food product used for seasoning in Central and Southern America. The present research aimed to investigate the chemical composition of an extract prepared from aerial parts of P. ruderale using UHPLC-DAD-MS/MS, to isolate and identify major natural products present in the extract, and to furtherly investigate their anti-inflammatory activity in vitro. Twenty-five compounds were detected and characterized using UV-Vis and MS data. All characterized compounds were quantified. Ten major phenolics were isolated and identified by NMR. One previously undescribed natural product was isolated and established as 1-O-(4-hydroxy-3,5-dimethoxy)benzoyl-6-O-galloyl-β-d-glucose (12). Anti-inflammatory activity was evaluated based on the influence of the extract and isolated compounds on the TLR4-dependent secretion of IL-8 and TNF-α by human primary neutrophils in vitro. Phenolic acids, and caffeic acid derivatives in particular, contributed to the extract's bioactivity.
刺果苏木亚种是中美洲和南美洲的一种调味食品。本研究旨在利用 UHPLC-DAD-MS/MS 分析刺果苏木地上部分提取物的化学成分，分离并鉴定提取物中的主要天然产物，并进一步研究其体外抗炎活性。通过 UV-Vis 和 MS 数据检测和表征了 25 种化合物。对所有鉴定的化合物进行了定量分析。通过 NMR 分离并鉴定了 10 种主要酚类化合物。分离并确定了一种以前未描述的天然产物，鉴定为 1-O-(4-羟基-3,5-二甲氧基)苯甲酰基-6-O-没食子酰基-β-D-葡萄糖（12）。根据提取物和分离化合物对人原代嗜中性粒细胞 TLR4 依赖性 IL-8 和 TNF-α分泌的影响，评估抗炎活性。酚酸，特别是咖啡酸衍生物，对提取物的生物活性有贡献。
