通过辣根过氧化物酶交联的基于海藻酸盐的水凝胶的制备及其在关节软骨工程中的应用。
Fabrication of alginate-based hydrogel cross-linked via horseradish peroxidase for articular cartilage engineering.
机构信息
Department of Tissue Engineering, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
Chemical Engineering Faculty, Sahand University of Technology, 51335-1996, Tabriz, Iran.
出版信息
BMC Res Notes. 2021 Sep 28;14(1):384. doi: 10.1186/s13104-021-05795-2.
OBJECTIVE
We aimed to detect the effect of a couple of parameters including Alg, HO, and HRP concentrations on the gelation time of Alg-based hydrogels using an enzymatic cross-linked procedure.
RESULTS
NMR, UV-Vis, and ATR-FTIR analyses confirmed the conjugation of Ph to the Alg backbone. Data showed gelation time was delayed with the increase and reduction of HO and HRP, respectively. We noted that hydrogel consisted of 1.2% (w/v) Alg, 5 U HRP, and 100 mM HO yielded an appropriate gelation time with appropriate mechanical properties. The addition of 0.5% (v/v) Col developed hydrogel increased the gelation time. The data showed that Alg, HRP, and HO with the ratio of 1:0.54:0.54 had proper physicochemical features for cartilage engineering.
目的
我们旨在检测 Alg、HO 和 HRP 浓度等参数对 Alg 基水凝胶的胶凝时间的影响，采用酶交联法。
结果
NMR、UV-Vis 和 ATR-FTIR 分析证实了 Ph 与 Alg 主链的连接。数据表明，HO 和 HRP 的增加和减少分别导致凝胶时间延长和缩短。我们注意到，含有 1.2%（w/v）Alg、5 U HRP 和 100 mM HO 的水凝胶具有适当的胶凝时间和机械性能。添加 0.5%（v/v）Col 的水凝胶可延长胶凝时间。数据表明，Alg、HRP 和 HO 的比例为 1:0.54:0.54 时具有用于软骨工程的适当理化特性。
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