Gantumur Enkhtuul, Sakai Shinji, Nakahata Masaki, Taya Masahito
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-cho, Toyonaka, Osaka 560-8531, Japan.
ACS Macro Lett. 2017 May 16;6(5):485-488. doi: 10.1021/acsmacrolett.7b00122. Epub 2017 Apr 12.
Hydrogels were obtained from aqueous solution containing polymer(s) possessing phenolic hydroxyl moieties through horseradish peroxidase (HRP)-catalyzed reaction without direct addition of HO. In this hydrogelation process, HO was generated from HRP and glucose contained in the aqueous solution, that is, HRP functioned not only as a catalyst, but also as a source of HO. The gelation time and mechanical properties of the resultant hydrogel could be altered by changing the concentrations of HRP and glucose. Cytocompatibility of the hydrogelation process was confirmed from cell studies using mouse 10T1/2 fibroblast cells.
水凝胶是通过辣根过氧化物酶（HRP）催化反应从含有具有酚羟基部分的聚合物的水溶液中获得的，无需直接添加H₂O₂。在这个水凝胶化过程中，H₂O₂由HRP和水溶液中含有的葡萄糖产生，也就是说，HRP不仅起到催化剂的作用，还作为H₂O₂的来源。通过改变HRP和葡萄糖的浓度，可以改变所得水凝胶的凝胶化时间和机械性能。使用小鼠10T1/2成纤维细胞进行的细胞研究证实了水凝胶化过程的细胞相容性。
