Sirowanto Ivanna, Josh Fonny, Zainuddin Andi Alfian, Faruk Muhammad
Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Plastic and Reconstructive Surgery Division, Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Ann Med Surg (Lond). 2021 Sep 8;70:102773. doi: 10.1016/j.amsu.2021.102773. eCollection 2021 Oct.
Stromal Vascular Fraction cells (SVFs) and Platelet Rich Plasma (PRP) are clinically proven to aid in cell regeneration and wound healing. The healing effects can be measured by the level of Epidermal Growth Factor (EGF). This study aims to investigate the effect of an SVFs and PRP combination on EGF levels in the anal trauma model.
Twenty-eight adult Wistar rats were divided into 3 groups: Group A consisted of healthy rats as a normal control group; Group B and C underwent modified anal surgical trauma and repair. Group B was treated with saline only and Group C was treated with local injection of a combination of SVFs and PRP after anal surgical repair. The EGF level was subsequently assessed on days 1, 7, and 14.
EGF levels were generally increased in Group C compared to Group B. A one-way ANOVA test result showed significantly increased EGF levels on days 7 (p = 0.038) and 14 (p = 0.018). Based on the linear regression test results, we found that local injection of PRP and SVFs after anal repair on an anal surgical trauma model can increase the EGF level in group C by 36.9% more than that of group B.
The combination of PRP and SVFs can increase the EGF level in the wound healing process of anal trauma. EGF is critical in the anal trauma healing process.
基质血管成分细胞（SVFs）和富血小板血浆（PRP）在临床上已被证明有助于细胞再生和伤口愈合。愈合效果可通过表皮生长因子（EGF）水平来衡量。本研究旨在探讨SVFs与PRP联合应用对肛门创伤模型中EGF水平的影响。
将28只成年Wistar大鼠分为3组：A组为健康大鼠作为正常对照组；B组和C组接受改良肛门手术创伤及修复。B组仅用生理盐水治疗，C组在肛门手术修复后局部注射SVFs与PRP的组合。随后在第1、7和14天评估EGF水平。
与B组相比，C组的EGF水平总体上有所升高。单向方差分析测试结果显示，在第7天（p = 0.038）和第14天（p = 0.018）EGF水平显著升高。基于线性回归测试结果，我们发现，在肛门手术创伤模型中，肛门修复后局部注射PRP和SVFs可使C组的EGF水平比B组提高36.9%。
PRP与SVFs的组合可提高肛门创伤伤口愈合过程中的EGF水平。EGF在肛门创伤愈合过程中至关重要。
