Platelet-rich plasma (PRP) and stromal vascular fraction (SVF) cells are clinically proven to aid in cellular regeneration and accelerate wound healing. The healing effect can be measured by epidermal growth factor (EGF) levels. This study aims to determine the effect of a combination of PRP and SVF injections on EGF levels during the healing of full-thickness burns in Wistar rats. Forty-eight adult Wistar rats were divided into 4 groups. Group A consisted of healthy rats. Groups B, C and D underwent modified full-thickness dermal burns. Group B was treated with local injections of PRP and SVF, Group C was treated with topical Vaseline, and Group D was treated with local injections of sterile water. EGF levels were subsequently assessed on days 1, 4, 7, 14 and 21 post-burn. EGF levels were generally increased in all groups, with the largest increase observed in the PRP and SVF injection group. A one-way ANOVA showed a significant increase in EGF levels on all days (p = <0.05). Based on the results of the linear regression test, local injection of PRP and SVF after full-thickness burns increases EGF levels by 27.3%. Combination PRP and SVF injections can increase EGF levels during the healing process of full-thickness burns. EGF is a critical growth factor in accelerating the healing process of full-thickness burns.