Raval Yash S, Mohamed Abdelrhman, Flurin Laure, Mandrekar Jayawant N, Greenwood Quaintance Kerryl E, Beyenal Haluk, Patel Robin
Division of Clinical Microbiology, Mayo Clinic, Rochester, MN, USA.
The Gene and Linda Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA, USA.
Biofilm. 2021 Sep 3;3:100055. doi: 10.1016/j.bioflm.2021.100055. eCollection 2021 Dec.
Biofilms formed by antibiotic-resistant bacteria in wound beds present unique challenges in terms of treating chronic wound infections; biofilms formed by one or more than one bacterial species are often involved. In this work, the anti-biofilm activity of a novel electrochemical bandage (e-bandage) composed of carbon fabric and controlled by a wearable potentiostat, designed to continuously deliver low amounts of hydrogen peroxide (HO) was evaluated against 34 mono-species and 12 dual-species membrane bacterial biofilms formed by , , , , , , , , , , and . Biofilms were grown on polycarbonate membranes placed atop agar plates. An e-bandage, which electrochemically reduces dissolved oxygen to HO when polarized at -0.6 V, was then placed atop each membrane biofilm and polarized continuously for 12, 24, and 48 h using a wearable potentiostat. Time-dependent decreases in viable CFU counts of all mono- and dual-species biofilms were observed after e-bandage treatment. 48 h of e-bandage treatment resulted in an average reduction of 8.17 ± 0.40 and 7.99 ± 0.32 log CFU/cm for mono- and dual-species biofilms, respectively. Results suggest that the described HO producing e-bandage can reduce viable cell counts of biofilms grown either in mono- or dual-species forms, and should be further developed as a potential antibiotic-free treatment strategy for treating chronic wound infections.
伤口床中由耐抗生素细菌形成的生物膜在治疗慢性伤口感染方面带来了独特的挑战；通常涉及由一种或多种细菌形成的生物膜。在这项工作中，评估了一种新型电化学绷带（电子绷带）的抗生物膜活性，该绷带由碳纤维织物组成，并由可穿戴恒电位仪控制，旨在持续释放少量过氧化氢（H₂O₂），其针对由金黄色葡萄球菌、表皮葡萄球菌、铜绿假单胞菌、大肠杆菌、肺炎克雷伯菌、鲍曼不动杆菌、阴沟肠杆菌、产气肠杆菌、奇异变形杆菌、粪肠球菌、屎肠球菌形成的34种单菌种和12种双菌种膜细菌生物膜进行了测试。生物膜生长在置于琼脂平板顶部的聚碳酸酯膜上。然后将一个在 -0.6 V极化时能将溶解氧电化学还原为H₂O₂的电子绷带放置在每个膜生物膜顶部，并使用可穿戴恒电位仪连续极化12、24和48小时。电子绷带处理后，观察到所有单菌种和双菌种生物膜的活菌CFU计数随时间下降。电子绷带处理48小时后，单菌种和双菌种生物膜的平均CFU/cm分别降低了8.17 ± 0.40和7.99 ± 0.32 log。结果表明，所述产生H₂O₂的电子绷带可以减少以单菌种或双菌种形式生长的生物膜的活菌数量，应进一步开发作为治疗慢性伤口感染的潜在无抗生素治疗策略。
