Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russian Federation.
Biofunctional Chemistry Laboratory, A. Butlerov Institute of Chemistry, Kazan Federal University, Kazan, Republic of Tatarstan, Russian Federation.
Sci Rep. 2020 Sep 9;10(1):14849. doi: 10.1038/s41598-020-71834-w.
In mixed infections, the bacterial susceptibility differs significantly compared to monocultures of bacteria, and generally the concentrations of antibiotics required for the treatment increases drastically. For S. aureus and P. aeruginosa dual species biofilms, it has been numerously reported that P. aeruginosa decreases S. aureus susceptibility to a broad range of antibiotics, including beta-lactams, glycopeptides, aminoglycosides, macrolides, while sensitizes to quinolones via secretion of various metabolites. Here we show that S. aureus also modulates the susceptibility of P. aeruginosa to antibiotics in mixed cultures. Thus, S. aureus-P. aeruginosa consortium was characterized by tenfold increase in susceptibility to ciprofloxacin and aminoglycosides compared to monocultures. The same effect could be also achieved by the addition of cell-free culture of S. aureus to P. aeruginosa biofilm. Moreover, similar increase in antibiotics efficacy could be observed following addition of S. aureus suspension to the P. aeruginosa mature biofilm, compared to P. aeruginosa monoculture, and vice versa. These findings open promising perspectives to increase the antimicrobial treatment efficacy of the wounds infected with nosocomial pathogens by the transplantation of the skin residential microflora.
在混合感染中，细菌的敏感性与细菌的单一培养物相比有显著差异，通常治疗所需的抗生素浓度会大幅增加。对于金黄色葡萄球菌和铜绿假单胞菌的双物种生物膜，已经有大量报道表明，铜绿假单胞菌降低了金黄色葡萄球菌对包括β-内酰胺类、糖肽类、氨基糖苷类、大环内酯类在内的多种抗生素的敏感性，同时通过分泌各种代谢物使金黄色葡萄球菌对喹诺酮类药物敏感。在这里，我们发现金黄色葡萄球菌也可以调节混合培养物中铜绿假单胞菌对抗生素的敏感性。因此，与单一培养物相比，金黄色葡萄球菌-铜绿假单胞菌联合体对环丙沙星和氨基糖苷类药物的敏感性增加了十倍。通过向铜绿假单胞菌生物膜中添加金黄色葡萄球菌无细胞培养液也可以达到相同的效果。此外，与铜绿假单胞菌单一培养物相比，将金黄色葡萄球菌混悬液添加到铜绿假单胞菌成熟生物膜中可以观察到抗生素疗效的类似增加，反之亦然。这些发现为通过移植皮肤常驻菌群来提高医院感染病原体感染伤口的抗菌治疗效果提供了有前景的思路。
