School of Pharmacy, Shenyang Key Laboratory of Functional Drug Carrier Materials, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenhe District, Shenyang, 110016, Liaoning Province, P. R. China.
Liaoning Province Center for Disease Control and Prevention, Shenyang, 110016, P. R. China.
Anal Methods. 2021 Oct 21;13(40):4805-4813. doi: 10.1039/d1ay01218e.
A simple, inexpensive, reliable and environmentally friendly method based on vortex-assisted dispersive liquid-liquid microextraction combined with solidification of a floating organic drop (VA-DLLME-SFO) followed by high performance liquid chromatography with a diode array detector (HPLC-DAD) was developed to determine benzoic acid (BA) and sorbic acid (SA) in ketchup and powder bags of instant noodles. Hydrophobic natural deep eutectic solvents (NADESs), as the microextraction solvent, were prepared with ternary components. Acetic acid can reduce the viscosity of hydrophobic NADESs and vortex can promote the dispersion of NADESs in aqueous solvents. In the DLLME process, several key parameters were optimized, including the type and volume of hydrophobic NADES, pH value, vortex time and salt content. Meanwhile, these parameters were assessed using response surface methodology based on the Box-Behnken design (BBD). Under optimal conditions, the developed method showed good linearity in the selected range (, 0.9997). The limits of detection were 0.2 μg mL and 0.07 μg mL for BA and SA, respectively. Recoveries were in the range of 82.21% to 102.70% and the relative standard deviations (intra-day and inter-day precisions) were less than 5.66%. The proposed method was successful in analyzing BA and SA in ketchup and powder bags of instant noodles.
建立了一种基于涡旋辅助分散液液微萃取结合漂浮有机液滴固化（VA-DLLME-SFO）的简单、经济、可靠且环保的方法，结合高效液相色谱二极管阵列检测（HPLC-DAD），用于测定番茄酱和方便面粉末包中的苯甲酸（BA）和山梨酸（SA）。疏水性天然深共熔溶剂（NADESs）作为微萃取溶剂，由三元成分制备。乙酸可以降低疏水性 NADES 的粘度，涡旋可以促进 NADES 在水相溶剂中的分散。在 DLLME 过程中，优化了几种关键参数，包括疏水性 NADES 的类型和体积、pH 值、涡旋时间和盐含量。同时，使用基于 Box-Behnken 设计（BBD）的响应面法评估了这些参数。在最佳条件下，该方法在所选择的范围内表现出良好的线性（，0.9997）。BA 和 SA 的检测限分别为 0.2 μg mL 和 0.07 μg mL。回收率在 82.21%至 102.70%之间，日内和日间精密度（相对标准偏差）小于 5.66%。该方法成功用于分析番茄酱和方便面粉末包中的 BA 和 SA。
