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多重耐药（MDR）和泛耐药（XDR）铜绿假单胞菌的毒力因子：一项对印度南部高发病区眼部感染分离株的横断面研究。

Virulence factors in multidrug (MDR) and Pan-drug resistant (XDR) Pseudomonas aeruginosa: a cross-sectional study of isolates recovered from ocular infections in a high-incidence setting in southern India.

作者信息

Naik Poonam, Pandey Suchita, Gagan Satyashree, Biswas Sudeshna, Joseph Joveeta

机构信息

Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Hyderabad, India.

Research Scholar, Manipal Academy of Higher Education, Manipal, India.

出版信息

J Ophthalmic Inflamm Infect. 2021 Sep 28;11(1):36. doi: 10.1186/s12348-021-00268-w.

DOI:10.1186/s12348-021-00268-w
PMID:34585284
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8479063/
Abstract

BACKGROUND

Global concerns have been raised due to upward trend of Multi-drug Resistant (MDR) Pseudomonas aeruginosa reports in ocular infections. Our aim was to characterize the virulence determinants of MDR P. aeruginosa causing ocular infections.

METHODS

P. aeruginosa strains were isolated from 46 patients with conjunctivitis (2), endophthalmitis (11) and active keratitis (25) seen at our Institute, between 2016 and 2020. The isolates were identified by Vitek-2 and characterized based on growth kinetics, biofilm formation, motility, pyoverdine and pyocyanin production, phospholipase and catalase activity, urease production along with expression of exotoxins (exo-A, exo-U and exo-S) and correlated to its antibiotic profiles.

RESULTS

Of the 46 P. aeruginosa isolates, 23 were MDR and were significantly (p = 0.03) associated with older (> 65) patients, along with higher production of pyoverdine (58.3%), pyocyanin (30.4%), phospholipase (91.6%) and protease (62.5%) activity, formed strong biofilms and exo-A (30.4%). No significant relation between motility, urease and catalase production with antibiotic susceptibility was observed. Heatmap and PCoA analysis confirmed this unique virulence profile associated with MDR-PA strains.

CONCLUSION

Phenotypic characteristics of P.aeruginosa might be responsible for increased colonization and antibiotic resistance observed in vivo and understanding these differences may lead to development of clinical guidelines for the management of MDR infections.

摘要

背景

由于眼部感染中多重耐药（MDR）铜绿假单胞菌报告呈上升趋势，引发了全球关注。我们的目的是对引起眼部感染的MDR铜绿假单胞菌的毒力决定因素进行表征。

方法

2016年至2020年期间，从我院就诊的46例结膜炎（2例）、眼内炎（11例）和活动性角膜炎（25例）患者中分离出铜绿假单胞菌菌株。通过Vitek-2鉴定分离株，并根据生长动力学、生物膜形成、运动性、绿脓菌素和绿脓青素产生、磷脂酶和过氧化氢酶活性、尿素酶产生以及外毒素（exo-A、exo-U和exo-S）表达进行表征，并将其与其抗生素谱相关联。

结果

在46株铜绿假单胞菌分离株中，23株为MDR，与老年（>65岁）患者显著相关（p = 0.03），同时绿脓菌素（58.3%）、绿脓青素（30.4%）、磷脂酶（91.6%）和蛋白酶（62.5%）活性产生较高，形成强生物膜和exo-A（30.4%）。未观察到运动性、尿素酶和过氧化氢酶产生与抗生素敏感性之间的显著关系。热图和主坐标分析证实了这种与MDR-PA菌株相关的独特毒力特征。

结论

铜绿假单胞菌的表型特征可能是体内观察到的定植增加和抗生素耐药性增加的原因，了解这些差异可能会导致制定MDR感染管理的临床指南。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/a757cac18992/12348_2021_268_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/826bd98c581d/12348_2021_268_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/0450810b4d4a/12348_2021_268_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/983f93c16cfa/12348_2021_268_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/30a83e091a40/12348_2021_268_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/64aee7c487f3/12348_2021_268_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/a757cac18992/12348_2021_268_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/826bd98c581d/12348_2021_268_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/0450810b4d4a/12348_2021_268_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/983f93c16cfa/12348_2021_268_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/30a83e091a40/12348_2021_268_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/64aee7c487f3/12348_2021_268_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a6c/8479063/a757cac18992/12348_2021_268_Fig6_HTML.jpg

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