Elkady Fathy M, Badr Bahaa M, Saied Ebrahim, Hashem Amr H, Abdel-Maksoud Mostafa A, Fatima Sabiha, Malik Abdul, Aufy Mohammed, Hussein Ahmed M, Abdulrahman Mohammed S, Hashem Hany R
Microbiology and Immunology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt.
Department of Basic Medical and Dental Sciences, Faculty of Dentistry, Zarqa University, Zarqa, Jordan.
Int J Nanomedicine. 2025 Apr 14;20:4705-4727. doi: 10.2147/IJN.S497494. eCollection 2025.
Clinical isolates of () are among the most recovered bacteria with phenotypic antimicrobial resistance. Bimetallic nanoparticles (BNPs) have received much attention for antimicrobial activity in the last decade. This research aimed to biosynthesize bimetallic copper oxide-selenium nanoparticles (CuO-Se BNPs) and to assess its bioactivity on various clinical isolates.
Based on the possible synergistic effects, CuO-Se BNPs were selected and biosynthesized using leaf extract of () for the first time. The obtained BNPs were characterized using UV-vis spectroscopy, X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), and transmission and scanning electron microscopes. The capability of Cu-Se BNPs to cease the growth of isolates and to reduce their virulence characters was evaluated. Also, different cell lines were used to assess its cytotoxicity and anticancer activity.
The elemental composition of CuO and Se was revealed by the UV, XRD, and EDX data, indicating the synthesis of CuO-Se core shell BNPs with a size of 50 nm. In well diffusion assay, CuO-Se BNPs growth with 10-21 mm inhibition zone diameter and 38-95% inhibition. Also, the minimum inhibitory concentration and minimum bactericidal concentration were in a relatively wide range of 7.8-250 μg/mL and 31.2-500 μg/mL, respectively, with tolerance level range of 2-16. Additionally, CuO-Se BNPs shown anti-pyocyanin activity of 4.35-63.21% inhibition while the anti-proteolytic activity was in a range of 4.96-12.59% and anti-pyoverdine effect was in a range of 0.24-83.41%. The IC50 against Wi-38 normal cells was 267.2 µg/mL while the IC50 were 31.1 and 83.4 µg/mL against MCF-7 and Hep-G2, respectively, indicating promising anticancer activity.
This research demonstrates the promising antibacterial, anti-virulence, and antitumor properties with safe low concentrations of CuO-Se NPs, synthesized via an eco-friendly green synthesis method without the use of toxic chemicals, offering a sustainable and cost-effective alternative.
（某种细菌名称未给出）的临床分离株是最常分离出的具有表型抗菌耐药性的细菌之一。在过去十年中，双金属纳米颗粒（BNPs）因其抗菌活性而备受关注。本研究旨在生物合成双金属氧化铜 - 硒纳米颗粒（CuO - Se BNPs）并评估其对各种临床分离株的生物活性。
基于可能的协同效应，首次选用（某种植物名称未给出）的叶提取物来生物合成CuO - Se BNPs。使用紫外 - 可见光谱、X射线衍射（XRD）、能量色散X射线光谱（EDX）以及透射和扫描电子显微镜对所得的BNPs进行表征。评估了Cu - Se BNPs抑制临床分离株生长以及降低其毒力特征的能力。此外，使用不同的细胞系评估其细胞毒性和抗癌活性。
紫外、XRD和EDX数据揭示了CuO和Se的元素组成，表明合成了尺寸为50nm的CuO - Se核壳BNPs。在平板扩散试验中，CuO - Se BNPs抑制（细菌名称未给出）生长，抑菌圈直径为10 - 21mm，抑制率为38 - 95%。此外，最低抑菌浓度和最低杀菌浓度分别在7.8 - 250μg/mL和31.2 - 500μg/mL的较宽范围内，耐受水平范围为2 - 16。另外，CuO - Se BNPs显示出4.35 - 63.21%的抑制绿脓菌素活性，而抗蛋白水解活性在4.96 - 12.59%范围内，抗绿脓菌素效应在0.24 - 83.41%范围内。对Wi - 38正常细胞的IC50为267.2μg/mL，而对MCF - 7和Hep - G2的IC50分别为31.1和83.4μg/mL，表明具有良好的抗癌活性。
本研究表明，通过环保的绿色合成方法合成的低浓度CuO - Se NPs具有良好的抗菌、抗毒力和抗肿瘤特性，且不使用有毒化学物质，提供了一种可持续且经济高效的替代方案。
