Busse Stefan, Hoffmann Jessica, Michler Enrico, Hartig Roland, Frodl Thomas, Busse Mandy
Department of Psychiatry, University of Magdeburg, Magdeburg, Germany.
Institute of Immunology, University of Magdeburg, Magdeburg, Germany.
Brain Behav Immun Health. 2021 Apr 20;14:100218. doi: 10.1016/j.bbih.2021.100218. eCollection 2021 Jul.
Inflammation and alterations in essential protein structures in the brain might also change the cellular distribution in the cerebrospinal fluid (CSF). Using flow cytometry, we analyzed cell populations of the innate and adaptive immune system associated with the most frequent forms of dementias. We included patients with mild cognitive impairment (MCI; N = 33), Alzheimer's disease (AD; N = 90), vascular dementia (VD; N = 35) and frontotemporal dementia (FTD; N = 17) at the time of diagnosis, before onset of treatment and 11 elderly non-demented individuals. Dependent on the form of dementia, an increased frequency of CD14 monocytes, NK cells and NKT cells was measured. Within the T cell population, a dementia-associated shift from central memory towards (late-stage) effector cells was detected. T cells and NKT cells were correlated with MMSE, NK and NKT cells were correlated with ptau, CD14 monocytes and NK cells were correlated with Amyloid-β 1-40. Our data suggest that each investigated immune cell type is involved in dementia-associated alterations within the CSF, possibly having distinct functions in their pathogenesis.
大脑中炎症和必需蛋白质结构的改变也可能会改变脑脊液（CSF）中的细胞分布。我们使用流式细胞术分析了与最常见痴呆形式相关的先天性和适应性免疫系统的细胞群。我们纳入了诊断时、治疗开始前的轻度认知障碍（MCI；n = 33）、阿尔茨海默病（AD；n = 90）、血管性痴呆（VD；n = 35）和额颞叶痴呆（FTD；n = 17）患者以及11名老年非痴呆个体。根据痴呆形式的不同，检测到CD14单核细胞、NK细胞和NKT细胞的频率增加。在T细胞群体中，检测到与痴呆相关的从中央记忆细胞向（晚期）效应细胞的转变。T细胞和NKT细胞与简易精神状态检查表（MMSE）相关，NK和NKT细胞与磷酸化tau蛋白（ptau）相关，CD14单核细胞和NK细胞与淀粉样β蛋白1-40（Amyloid-β 1-40）相关。我们的数据表明，每种被研究的免疫细胞类型都参与了脑脊液中与痴呆相关的改变，可能在其发病机制中具有不同的功能。
