Korea Atomic Energy Research Institute, 29, Geumgu-gil, Jeongeup-si, Jeollabuk-do, 56212, Republic of Korea.
J Mater Chem B. 2021 Oct 13;9(39):8237-8245. doi: 10.1039/d1tb01473k.
Nano-sized materials with properties that enable their internalization into target cells using passive targeting systems have been utilized with radioisotopes to track their pharmacokinetics in the body. Here, we report the incorporation of novel chelator-free Zr-89 using a hierarchical iron oxide nanocomposite (Zr-IONC). Characterization revealed that it had a rice-shape with a mean width of 160 nm. The surface of the Zr-IONCs was coated by polyethyleneimine (PEI) and polyvinylpyrrolidone (PVP) to improve the cancer target efficacy. The biological behavior of the nanoparticles coated with the polymers differed significantly by the surface composition. Positron emission tomography measurements by the labeled Zr-89 effectively confirmed the cancer target capability and the fate of distribution in the body. We found that only PVP coated Zr-IONC reached the tumor region while non-coated and PEI coated Zr-IONC tended to be undesirably entirely cleared in the liver and spleen. The Zr-incorporated iron oxide nanocomposite is significantly stable for radiolabeling despite various surface modifications, allowing the potential carrier to specifically target cancer cells. The strategy of utilizing the biocompatible PEI and PVP surface coating system for negative charged nanoparticles such as iron oxide will afford enhanced biological application.
具有使它们能够通过被动靶向系统进入靶细胞的特性的纳米级材料已与放射性同位素一起用于跟踪其在体内的药代动力学。在这里，我们报告了使用分层氧化铁纳米复合材料（Zr-IONC）来掺入新型无螯合剂 Zr-89。表征表明，它具有平均宽度为 160nm 的稻形状。Zr-IONC 的表面涂覆有聚乙烯亚胺（PEI）和聚乙烯吡咯烷酮（PVP），以提高癌症靶向功效。涂覆有聚合物的纳米粒子的生物行为因表面组成而有很大差异。标记的 Zr-89 的正电子发射断层扫描测量有效地证实了癌症靶标能力以及在体内的分布命运。我们发现，只有 PVP 涂覆的 Zr-IONC 到达肿瘤区域，而未涂覆和涂覆有 PEI 的 Zr-IONC 倾向于在肝脏和脾脏中被完全清除。尽管进行了各种表面修饰，但掺入 Zr 的氧化铁纳米复合材料对于放射性标记非常稳定，允许潜在的载体特异性靶向癌细胞。利用生物相容性的 PEI 和 PVP 表面涂层系统对负电荷纳米粒子（如氧化铁）的策略将提供增强的生物应用。
