Donnerer J, Oka K, Brossi A, Rice K C, Spector S
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4566-7. doi: 10.1073/pnas.83.12.4566.
Endogenous codeine and morphine were identified in rat brain by immunological determination following HPLC. To demonstrate occurrence of a biosynthetic pathway to morphine in mammals similar to that used by the poppy plant, (+)-salutaridine, (-)-thebaine, and (-)-codeine were administered to rats intravenously. These compounds, which are intermediates in the synthesis of morphine in Papaver somniferum, caused a marked increase in the codeine and morphine levels in rat tissues. This provides evidence for a biosynthetic pathway to morphine in mammalians.
通过高效液相色谱法（HPLC）后的免疫测定法，在大鼠脑中鉴定出内源性可待因和吗啡。为了证明哺乳动物中存在一条类似于罂粟植物所使用的吗啡生物合成途径，将（+）-氢可酮、（-）-蒂巴因和（-）-可待因静脉注射给大鼠。这些化合物是罂粟中吗啡合成的中间体，导致大鼠组织中可待因和吗啡水平显著升高。这为哺乳动物中吗啡的生物合成途径提供了证据。
