College of Public Health, Zhengzhou University, Zhengzhou, 450001, China.
School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China.
Biosens Bioelectron. 2022 Jan 1;195:113661. doi: 10.1016/j.bios.2021.113661. Epub 2021 Sep 25.
Aptamer-based dual recognition strategy, using dual aptamers or the cooperation of aptamers with other recognition elements, can better utilize the advantages of each recognition molecule and increase the design flexibility to effectively overcome the limitations of a single molecule recognition strategy, thereby improving the sensitivity and selectivity and facilitating the regulation of biological process. Hence, this review systematically tracks the construction and application of dual aptamers recognition strategy in the versatile detection of protein biomarkers, pathogenic microorganisms, cancer cells, and the treatment of some diseases and, more importantly, in functional regulation and imaging of cell-surface protein receptors. Then, the cooperation of aptamers with other recognition elements are briefly introduced. Potential challenges facing this field have been highlighted, aiming to expand bioanalytical applications of aptamer-based dual or multiple recognition strategies and meet the growing demand for precision medicine.
基于适配体的双重识别策略，使用双重适配体或适配体与其他识别元件的协同作用，可以更好地利用每种识别分子的优势，并增加设计灵活性，从而有效地克服单一分子识别策略的局限性，提高灵敏度和选择性，并促进对生物过程的调控。因此，本综述系统地跟踪了双重适配体识别策略在蛋白质生物标志物、致病微生物、癌细胞的多功能检测中的构建和应用，更重要的是，还跟踪了其在细胞表面蛋白受体的功能调控和成像中的应用。然后，简要介绍了适配体与其他识别元件的协同作用。突出了该领域面临的潜在挑战，旨在扩大基于适配体的双重或多重识别策略的生物分析应用，以满足精准医学日益增长的需求。
