利用电穿孔法将DNA介导的基因导入表皮细胞。

DNA-mediated gene transfer into epidermal cells using electroporation.

作者信息

Reiss M, Jastreboff M M, Bertino J R, Narayanan R

出版信息

Biochem Biophys Res Commun. 1986 May 29;137(1):244-9. doi: 10.1016/0006-291x(86)91202-7.

Abstract

A reliable method for the introduction of foreign DNA into epidermal cells is described. Electroporation of murine BALB/c MK-1 epidermal cells with pSV2-CAT resulted in the transient expression of chloramphenicol-acetyltransferase (0.03 to 0.05 nmoles acetylchloramphenicol per mg protein per min) in the transfected cells. Transfection of MK-1 cells with pSV2-neo led to the appearance of approximately eight G418 resistant clones per 10(-6) cells per microgram of plasmid DNA. Distinct patterns of integration of SV2-neo were detected in three different resistant clones.

摘要

本文描述了一种将外源DNA导入表皮细胞的可靠方法。用pSV2-CAT对小鼠BALB/c MK-1表皮细胞进行电穿孔，导致转染细胞中氯霉素乙酰转移酶的瞬时表达（每分钟每毫克蛋白质0.03至0.05纳摩尔乙酰氯霉素）。用pSV2-neo转染MK-1细胞，每微克质粒DNA每10(-6)个细胞中出现约8个对G418抗性的克隆。在三个不同的抗性克隆中检测到SV2-neo的不同整合模式。