用于通过DNA高效转染哺乳动物细胞的电穿孔法。
Electroporation for the efficient transfection of mammalian cells with DNA.
作者信息
Chu G, Hayakawa H, Berg P
出版信息
Nucleic Acids Res. 1987 Feb 11;15(3):1311-26. doi: 10.1093/nar/15.3.1311.
Abstract
A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.
摘要
本文描述了一种通过电穿孔将DNA导入哺乳动物细胞的简单且可重复的方法。研究了涉及细胞、DNA和电场的参数。该方法已应用于广泛的动物细胞。它能够将超过1%的活细胞转化为稳定表达可选择标记物的细胞。
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