Pecoraro G, Morgan D, Defendi V
Department of Pathology and Kaplan Cancer Center, New York University Medical Center, NY 10016.
Proc Natl Acad Sci U S A. 1989 Jan;86(2):563-7. doi: 10.1073/pnas.86.2.563.
The human papillomaviruses (HPVs) are associated with specific benign and malignant lesions of the skin and mucosal epithelia. Cloned viral DNAs from HPV types 6b, 16, and 18 associated with different pathological manifestations of genital neoplasia in vivo were introduced into primary human cervical epithelial cells by electroporation. Cells transfected with HPV16 or HPV18 DNA acquired indefinite lifespans, distinct morphological alterations, and anchorage-independent growth (HPV18), and contain integrated transcriptionally active viral genomes. HPV6b or plasmid electroporated cells senesced at low passage. The alterations in growth and differentiation of the cells appear to reflect the progressive oncogenic processes that result in cervical carcinoma in vivo.
人乳头瘤病毒（HPV）与皮肤和黏膜上皮的特定良性及恶性病变相关。通过电穿孔法将来自与体内生殖器肿瘤不同病理表现相关的HPV 6b型、16型和18型的克隆病毒DNA导入原代人宫颈上皮细胞。用HPV16或HPV18 DNA转染的细胞获得了无限寿命、明显的形态改变以及不依赖贴壁生长的特性（HPV18），并且含有整合的转录活性病毒基因组。经HPV6b或质粒电穿孔的细胞在传代次数较低时就会衰老。细胞生长和分化的改变似乎反映了在体内导致宫颈癌的渐进性致癌过程。
