Mousa Nuha M, Jasim Hameed M
College of Science, Al-Muthana University, Iraq.
College of Biotechnology, Al-Nahrain University, Iraq.
Ann Parasitol. 2021;67(2):281-286. doi: 10.17420/ap6702.340.
Toxoplasma gondii infections are endemic in Iraq and represent serious problems. Human toxoplasmosis can be associated with serious clinical manifestations, particularly in developing fetus. This study was aimed to determine the distribution of genotypes and alleles, residing within interleukin-6 (IL-6) and interleukin-1 beta (IL-1β) polymorphisms, among fetuses and neonates, congenitally infected with Toxoplasma gondii, and among uninfected control cases. Blood samples were collected from 125 aborted women with a history of single or recurrent miscarriage, in addition to fifty normal healthy control women. Molecular identification of the parasite was performed by detecting Toxoplasma B1 gene using real-time qPCR technique. IL-6 and IL-1β gene expression was assayed in each case-study samples by using RT-PCR. T. gondii was detected in recurrent toxoplasmosis aborted women at percent (16%). IL-1β and IL-6 gene expression was significantly increased in toxoplasmosis women compared with healthy control women. Fold expression of IL-1β was 9.5 in toxoplasmosis patients compared with one fold in healthy control. IL-1β and IL-6 over-expression was correlated to the high-risk toxoplasmosis infection and could be a biomarker for prognosis of the disease.
弓形虫感染在伊拉克呈地方性流行，是严重问题。人类弓形虫病可能伴有严重临床表现，尤其是在发育中的胎儿身上。本研究旨在确定在先天性感染弓形虫的胎儿和新生儿以及未感染的对照病例中，白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）多态性中的基因型和等位基因分布。除了50名正常健康对照女性外，还从125名有单次或复发性流产史的流产女性中采集了血样。使用实时定量PCR技术通过检测弓形虫B1基因进行寄生虫的分子鉴定。通过逆转录聚合酶链反应（RT-PCR）检测每个病例研究样本中的IL-6和IL-1β基因表达。在复发性弓形虫病流产女性中检测到弓形虫的比例为16%。与健康对照女性相比，弓形虫病女性中IL-1β和IL-6基因表达显著增加。弓形虫病患者中IL-1β的表达倍数为9.5，而健康对照中为1倍。IL-1β和IL-6的过度表达与高危弓形虫病感染相关，可能是该疾病预后的生物标志物。
