Postgraduate student, Biomaterials and Technology, Department of Reconstructive Dentistry, University Center for Dental Medicine Basel, Basel, Switzerland.
Assistant Professor, Senior consultant Oral and Cranio-Maxillofacial Surgery / Co-director 3D Print Lab, University Hospital Basel, Basel, Switzerland; and Head of Medical Additive Manufacturing Research Group (Swiss MAM), Department of Biomedical Engineering, University of Basel, Allschwil, Switzerland.
J Prosthet Dent. 2023 Jun;129(6):939-945. doi: 10.1016/j.prosdent.2021.08.015. Epub 2021 Sep 29.
Occlusal devices can be either conventionally processed, milled, or printed. However, little is known about the biocompatibility of 3D printing resin materials.
The purpose of this in vitro study was to compare the viability and morphology of human gingival fibroblast cells (HFG-1) after cultivation on conventionally processed, milled, and printed occlusal device materials with different surface treatments.
Disks of a conventionally processed (PalaXpress Clear [pP]), milled (Yamahachi PMMA Clear [sY]), and 2 different printed materials (Dental LT Clear Resin [aD]; Freeprint splint [aF]) were prepared. The surfaces of the specimens were finished by using 2 different treatments (unpolished and polished with P1200-grit silicon carbide paper). HGF-1 cells were cultivated on the specimens for 24 hours, and a viability assay was performed by using polystyrene disks as a control (n=9 disks per group). Cell morphology and the topography of the specimens were examined with scanning electron microscopy (n=3 disks per group). Two-way analysis of variance was applied to determine the effect of material and surface treatment followed by the post hoc Fisher least significant difference test (α=.05).
Overall, material (P<.001) and surface treatment (P<.001) significantly influenced the viability of HGF-1 cells. The viability of cells on all specimens displayed mean values between 0.85 and 1.01 compared with the control except for unpolished aD (0.00 ±0.07) and aF (0.02 ±0.05) that had only a few cells with a round shape.
The behavior of HGF-1 cells on conventionally processed and milled specimens was similar and not dependent on the surface treatment. Unpolished printed specimens had a cytotoxic effect. However, after polishing, cell behavior was similar to that of the conventionally processed and milled specimens.
咬合装置可以通过常规加工、铣削或打印来制作。然而，对于 3D 打印树脂材料的生物相容性知之甚少。
本体外研究的目的是比较在经过不同表面处理的常规加工、铣削和打印的咬合装置材料上培养后，人牙龈成纤维细胞（HFG-1）的活力和形态。
制备了常规加工（PalaXpress Clear [pP]）、铣削（Yamahachi PMMA Clear [sY]）和 2 种不同打印材料（Dental LT Clear Resin [aD]；Freeprint splint [aF]）的圆盘。使用 2 种不同的处理方法（未抛光和用 P1200 粒度碳化硅砂纸抛光）对试件表面进行处理。将 HGF-1 细胞培养在试件上 24 小时，并用聚苯乙烯圆盘作为对照进行活力测定（每组 9 个圆盘）。用扫描电子显微镜检查细胞形态和试件形貌（每组 3 个圆盘）。采用双因素方差分析确定材料和表面处理的影响，然后采用事后 Fisher 最小显著差异检验（α=.05）。
总体而言，材料（P<.001）和表面处理（P<.001）显著影响 HGF-1 细胞的活力。除了未抛光的 aD（0.00±0.07）和 aF（0.02±0.05）外，所有试件上细胞的活力值均在 0.85 到 1.01 之间，与对照相比，这些试件只有少数细胞呈圆形。
HGF-1 细胞在常规加工和铣削试件上的行为相似，且不依赖于表面处理。未抛光的打印试件具有细胞毒性作用。然而，经过抛光后，细胞行为与常规加工和铣削试件相似。
