Lin Hui, Wang Pengfei, Zhang Wanhong, Yan Hongwang, Yu Hongxi, Yan Lingqiao, Chen Hui, Xie Mindan, Shan Liqun
Department of Thoracic Surgery, First People's Hospital of Wenling, Wenling, 317500, People's Republic of China.
Department of Neurosurgery, The Kaifeng Central Hospital of Xinxiang Medical University, Kaifeng, 475000, People's Republic of China.
Inflammation. 2022 Feb;45(1):428-444. doi: 10.1007/s10753-021-01556-2. Epub 2021 Oct 2.
A new method for targeting lung infections is of great interest using biodegradable nanoparticles. In this study, bergenin-loaded BSA NPs were developed against lung injury. Briefly, bergenin-loaded bovine serum albumin nanoparticles (BG@BSA NPs) were synthesized and characterized. HPLC recorded the major peak of bergenin. UV-Vis spectra had an absorbance at 376 nm. XRD revealed the presence of crystalline particles. FTIR confirmed the occurrence of functionalized molecules in the synthesized NPs. The particles were highly stable with a net negative charge of - 24.2. The morphology of NPs was determined by SEM and TEM. The mean particle size was 124.26 nm. The production of NO by NR8383 cells was decreased by BG@BSA NPs. Also, in mice, lipopolysaccharide-mediated acute lung inflammation was induced. BG@BSA NPs reduced macrophages and neutrophils in BALF and remarkably enhanced wet weight-to-dry weight (W/D) ratios and myeloperoxidase (MPO) activity. Further, BG@BSA NPs inhibited the production of inflammatory cells as well as tumor necrosis factor. The histopathological studies revealed that the damage and neutrophil infiltration were greatly inhibited by BG@BSA NPs. This indicates that BG@BSA NPs may be used to treat lung infections. Therefore, this study has given new insight into producing an active drug for the treatment of lung-associated diseases in the future.
利用可生物降解的纳米颗粒靶向肺部感染的新方法备受关注。在本研究中，开发了负载岩白菜素的牛血清白蛋白纳米颗粒用于对抗肺损伤。简而言之，合成并表征了负载岩白菜素的牛血清白蛋白纳米颗粒（BG@BSA NPs）。高效液相色谱法记录了岩白菜素的主峰。紫外可见光谱在376 nm处有吸光度。X射线衍射显示存在结晶颗粒。傅里叶变换红外光谱证实了合成纳米颗粒中功能化分子的存在。这些颗粒高度稳定，净负电荷为-24.2。通过扫描电子显微镜和透射电子显微镜确定了纳米颗粒的形态。平均粒径为124.26 nm。BG@BSA NPs降低了NR8383细胞产生的一氧化氮。此外，在小鼠中，诱导了脂多糖介导的急性肺部炎症。BG@BSA NPs减少了支气管肺泡灌洗液中的巨噬细胞和中性粒细胞，并显著提高了湿重与干重（W/D）比值和髓过氧化物酶（MPO）活性。此外，BG@BSA NPs抑制了炎症细胞以及肿瘤坏死因子的产生。组织病理学研究表明，BG@BSA NPs极大地抑制了损伤和中性粒细胞浸润。这表明BG@BSA NPs可用于治疗肺部感染。因此，本研究为未来生产用于治疗肺部相关疾病的活性药物提供了新的见解。
