Centro de Biología Molecular "Severo Ochoa," CSIC and Universidad Autónoma de Madrid, Madrid 28049, Spain.
G3 (Bethesda). 2021 Dec 8;11(12). doi: 10.1093/g3journal/jkab348.
Protein kinases and phosphatases constitute a large family of conserved enzymes that control a variety of biological processes by regulating the phosphorylation state of target proteins. They play fundamental regulatory roles during cell cycle progression and signaling, among other key aspects of multicellular development. The complement of protein kinases and phosphatases includes approximately 326 members in Drosophila, and they have been the subject of several functional screens searching for novel components of signaling pathways and regulators of cell division and survival. These approaches have been carried out mostly in cell cultures using RNA interference to evaluate the contribution of each protein in different functional assays and have contributed significantly to assign specific roles to the corresponding genes. In this work, we describe the results of an evaluation of the Drosophila complement of kinases and phosphatases using the wing as a system to identify their functional requirements in vivo. We also describe the results of several modifying screens aiming to identify among the set of protein kinases and phosphatases additional components or regulators of the activities of the epidermal growth factor and insulin receptors signaling pathways.
蛋白激酶和磷酸酶是一大类保守酶，通过调节靶蛋白的磷酸化状态来控制多种生物过程。它们在细胞周期进程和信号转导等多细胞发育的关键方面发挥基本的调节作用。在果蝇中，蛋白激酶和磷酸酶的复合物大约包含 326 个成员，它们一直是寻找信号通路新成分和细胞分裂与存活调节剂的几个功能筛选的主题。这些方法主要在细胞培养物中使用 RNA 干扰来评估每个蛋白在不同功能测定中的贡献，并为特定基因赋予特定作用做出了重大贡献。在这项工作中，我们描述了使用翅膀作为系统评估果蝇激酶和磷酸酶复合物的结果，以鉴定它们在体内的功能需求。我们还描述了几个修饰筛选的结果，旨在从蛋白激酶和磷酸酶组中鉴定表皮生长因子和胰岛素受体信号通路活性的其他成分或调节剂。
