State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China.
Food Chem. 2022 Mar 1;371:131145. doi: 10.1016/j.foodchem.2021.131145. Epub 2021 Sep 15.
Zearalenone (ZEN) poses a serious threat to human and animal health. The development of sensitive determination methods for ZEN is of great significance for ensuring the quality and safety of food. Herein, based on a stimuli-responsive aptamer-functionalized metal-organic framework (MOF) nano-container and trivalent DNA peroxidase mimicking enzyme (DNAzyme), an efficient aptasensor was constructed initially for the colorimetric determination of ZEN. The proposed aptasensor only required simple operations but exhibited outstanding specificity, reproducibility, storage stability and reusability simultaneously. Under the optimal conditions, there was a good linear relationship between the changed absorbance and logarithm concentration of ZEN within 0.01-100 ng mL, and the limit of detection (LOD) could reach 0.36 pg mL. Moreover, the proposed aptasensor was reliable in quantifying ZEN in spiked food samples. The current bioassay provides a promising scheme for constructing stable, specific and rapid colorimetric platforms with potential applications in the fields of food safety.
玉米赤霉烯酮（ZEN）对人类和动物健康构成严重威胁。开发用于 ZEN 的灵敏测定方法对于确保食品的质量和安全具有重要意义。在此，基于对刺激有响应的适体功能化金属有机骨架（MOF）纳米容器和三价 DNA 过氧化物酶模拟酶（DNAzyme），我们首次构建了一种用于 ZEN 比色测定的高效适体传感器。该适体传感器仅需要简单的操作，但同时具有出色的特异性、重现性、储存稳定性和可重复性。在最佳条件下，ZEN 的对数浓度与吸光度变化之间存在良好的线性关系，检测限（LOD）可达到 0.36pgmL。此外，该适体传感器可用于定量测定加标食品样品中的 ZEN。本生物测定法为构建稳定、特异和快速的比色平台提供了有前景的方案，有望在食品安全领域得到应用。
