State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Key Laboratory of Meat Processing of Sichuan, Chengdu University, Chengdu 610106, China.
Food Chem. 2023 Mar 1;403:134242. doi: 10.1016/j.foodchem.2022.134242. Epub 2022 Sep 15.
Fumonisin B (FB), the most occurring fumonisin, is capable of disrupting sphingolipid metabolism and poses a serious threat to human and animal health. The high incidence and toxicity of FB have created an urgent need for alternative quantitative determination strategies. Herein, a colorimetric aptasensor was developed by combining the catalytical metal-organic frameworks with the polyacrylamide-DNA hydrogel. The porphyrin-based MOF provided the aptasensor with robust peroxidase-like activity while maintaining good stability. Concurrently, the polyacrylamide-DNA hydrogel constructed via strand-induced hybridization chain reaction (HCR) endowed the aptasensor with outstanding stimuli-responsiveness towards FB molecules. The developed aptasensor demonstrated remarkable selectivity and ease of use for assays conducted in solution and food samples. It also exhibited superior analytical performance with a linear range of 0.05-100 ng mL and a limit of detection (LOD) of 0.024 ng mL. The strategy could serve as a model to develop diverse stimuli-responsive aptasensors targeting hazards in food safety.
伏马菌素 B（FB）是最常见的伏马菌素，能够破坏神经鞘脂代谢，对人类和动物健康构成严重威胁。FB 的高发生率和毒性促使人们迫切需要替代的定量测定策略。本研究通过将催化金属有机骨架与聚丙烯酰胺-DNA 水凝胶相结合，开发了一种比色适体传感器。基于卟啉的 MOF 为适体传感器提供了强大的过氧化物酶样活性，同时保持了良好的稳定性。同时，通过链诱导杂交链式反应（HCR）构建的聚丙烯酰胺-DNA 水凝胶使适体传感器对 FB 分子具有出色的刺激响应性。该方法在溶液和食品样品中的检测具有良好的选择性和易用性，表现出优异的分析性能，线性范围为 0.05-100ngmL，检测限（LOD）为 0.024ngmL。该策略可以作为开发针对食品安全危害的多种刺激响应适体传感器的模型。
