College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China.
College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710119, China; The Engineering Research Center for High-Valued Utilization of Fruit Resources in Western China, Ministry of Education, Xi'an 710119, China.
Food Chem. 2024 Jan 15;431:136908. doi: 10.1016/j.foodchem.2023.136908. Epub 2023 Aug 3.
An efficient aptasensor was developed for the colorimetric determination of zearalenone (ZEN) based on polyvalent aptamer scaffold and light-responsive oxidase-like nanozyme. The sensitivity and efficiency of the development method were significantly improved owing to rich aptamers and signal labels (3, 4-dihydroxybenzoic acid, PCA) packed in the scaffold. The scaffold integrated functions of target recognition, surface immobilization and signal transduction. The photoresponsive nanoenzyme of TiO-PCA was formed by PCA coordinated with Ti (IV) on the surface of TiO. TiO-PCA catalyzed dissolved oxygen rather than HO to generate colorimetric signal by stimulating the chromogenic substrate, which made the assay greener and safer. The detection limit of colorimetric mode was 0.0087 ng/mL and the satisfactory recoveries 92.00 %-111.00 % were achieved in spiked food samples. This strategy opens new horizons for sensitive detection of small molecule hazards and promises to be a powerful tool for safeguarding food safety.
一种基于多价适体支架和光响应氧化酶样纳米酶的比色法测定玉米赤霉烯酮 (ZEN) 的适体传感器被开发出来。由于支架中富含适体和信号标签（3,4-二羟基苯甲酸、PCA），开发方法的灵敏度和效率得到了显著提高。支架集成了目标识别、表面固定化和信号转导功能。TiO-PCA 的光响应纳米酶是由 PCA 与 TiO 表面的 Ti（IV）配位形成的。TiO-PCA 通过刺激显色底物，利用溶解氧而不是 HO 生成比色信号，使测定更加环保和安全。比色模式的检测限为 0.0087ng/mL，在添加的食品样品中获得了 92.00%-111.00%的满意回收率。该策略为小分子危害的灵敏检测开辟了新的视野，有望成为保障食品安全的有力工具。
