Kolb E
Z Gesamte Inn Med. 1986 May 1;41(9):249-55.
The clarification of the structure and the function of deoxyribonucleic acids with hereditary properties (genes) has led to the development of methods for their biochemical and chemical synthesis, respectively. Such synthetic genes may be introduced in bacterial cells with the help of carrier-DNA (vectors, e.g. plasmid- or bacteriophage-DNA) and may be used for the production of certain hormones (proinsulin, growth hormone), medicaments (interferons) and vaccines (e.g. covering proteins of viruses and of peptides from such ones respectively. Genes may also be introduced into the pronuclei of the zygote of mammals. Then in the germs, however, appear considerable losses. The creation of descendants with the same hereditary factors is possible in mammals up to now only by the dismemberment of the germ up to the eight-cell-stage. It is referred to ethical aspects of the use of gene-technical methods. Several problems in the application of biotechnical techniques in the reproduction are described.
对具有遗传特性（基因）的脱氧核糖核酸的结构和功能的阐明，分别导致了其生化合成方法和化学合成方法的发展。这种合成基因可借助载体DNA（载体，如质粒或噬菌体DNA）导入细菌细胞，并可用于生产某些激素（胰岛素原、生长激素）、药物（干扰素）和疫苗（例如分别覆盖病毒蛋白和此类病毒的肽）。基因也可导入哺乳动物受精卵的原核中。然而，在生殖细胞中会出现相当大的损失。到目前为止，在哺乳动物中，只有将生殖细胞分割到八细胞阶段，才有可能创造出具有相同遗传因子的后代。这涉及到基因技术方法使用的伦理问题。文中描述了生物技术在生殖应用中的几个问题。
