Assistant, Department of General and Medical Genetics, Institute of Biology and Biomedicine; National Research Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, Nizhny Novgorod, 603950, Russia.
Junior Researcher, Department of Applied Mathematics, Institute of Information Technologies, Mathematics and Mechanics; National Research Lobachevsky State University of Nizhni Novgorod, 23 Prospekt Gagarina, Nizhny Novgorod, 603950, Russia.
Sovrem Tekhnologii Med. 2021;13(3):26-31. doi: 10.17691/stm2021.13.3.03. Epub 2021 Jun 28.
was to conduct a functional analysis of sex-specific age-related changes in DNA methylation.
The study used a GSE87571 methylation dataset obtained from the blood DNA of 729 individuals aged 14 to 94 using the Illumina Infinium HumanMethylation450K BeadChip (USA). Gene ontology analysis was performed for 3 groups of genes (females, males, and duplicates) using the PANTHER database. The DAVID platform was used to perform KEGG metabolic pathway analysis.
The studies revealed unique for males and females changes in methylation of CpG sites, associated with certain metabolic processes. It was demonstrated that most of the CpG sites, for which methylation changes with age were revealed in both sexes, are associated with the genes responsible for the development and functioning of the nervous system. In males, unique age-related methylation changes affect CpG sites associated with changes in the immune system and lipid metabolism. In females, most CpGs are associated with changes involved in transcription and translation processes. Analysis of biological functions by KEGG revealed that a unique process associated with age-related changes in methylation of the glutamatergic system is typical for males. In females, unique biological processes with age-related changes include genes responsible for the development of diabetes and genes associated with cAMP signaling cascades (KEGG:04024).
Our studies reveal fundamental features of sex-dependent changes in methylation of CpG sites with variance increasing, which may indicate differences in age-related changes.
本研究旨在对性别特异性与年龄相关的 DNA 甲基化变化进行功能分析。
本研究使用了从 729 名年龄在 14 至 94 岁的个体的血液 DNA 中获得的 GSE87571 甲基化数据集，该数据集使用了 Illumina Infinium HumanMethylation450K BeadChip（美国）。使用 PANTHER 数据库对 3 组基因（女性、男性和重复）进行了基因本体论分析。使用 DAVID 平台进行了 KEGG 代谢途径分析。
本研究揭示了与某些代谢过程相关的 CpG 位点甲基化在男性和女性中独特的随年龄变化的情况。研究表明，大多数与年龄相关的甲基化变化相关的 CpG 位点，这些变化在两性中都被揭示出来，与负责神经系统发育和功能的基因有关。在男性中，与免疫系统和脂质代谢变化相关的 CpG 位点的独特年龄相关的甲基化变化会影响。在女性中，大多数 CpG 与涉及转录和翻译过程的变化有关。KEGG 的生物功能分析表明，与谷氨酸能系统的年龄相关的甲基化变化相关的独特过程是男性特有的。在女性中，与年龄相关的变化有关的独特生物学过程包括与糖尿病发展有关的基因和与 cAMP 信号级联有关的基因（KEGG：04024）。
我们的研究揭示了 CpG 位点甲基化性别依赖性变化的基本特征，其方差增加，这可能表明与年龄相关的变化存在差异。
