用于即时检测：一种更亮的溶剂致变色探针可在数分钟内检测出分枝杆菌。

Toward Point-of-Care Detection of : A Brighter Solvatochromic Probe Detects Mycobacteria within Minutes.

作者信息

Kamariza Mireille, Keyser Samantha G L, Utz Ashley, Knapp Benjamin D, Ealand Christopher, Ahn Green, Cambier C J, Chen Teresia, Kana Bavesh, Huang Kerwyn Casey, Bertozzi Carolyn R

机构信息

Department of Biology, Stanford University, Stanford, California 94305, United States.

Department of Chemistry, University of California, Berkeley, Berkeley, California 94720, United States.

出版信息

JACS Au. 2021 Jul 26;1(9):1368-1379. doi: 10.1021/jacsau.1c00173. eCollection 2021 Sep 27.

DOI:10.1021/jacsau.1c00173

PMID:34604847

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8479770/

Abstract

There is an urgent need for point-of-care tuberculosis (TB) diagnostic methods that are fast, inexpensive, and operationally simple. Here, we report on a bright solvatochromic dye trehalose conjugate that specifically detects (Mtb) in minutes. 3-Hydroxychromone (3HC) dyes, known for having high fluorescence quantum yields, exhibit shifts in fluorescence intensity in response to changes in environmental polarity. We synthesized two analogs of 3HC-trehalose conjugates (3HC-2-Tre and 3HC-3-Tre) and determined that 3HC-3-Tre has exceptionally favorable properties for Mtb detection. 3HC-3-Tre-labeled mycobacterial cells displayed a 10-fold increase in fluorescence intensity compared to our previous reports on the dye 4,4--dimethylaminonapthalimide (DMN-Tre). Excitingly, we detected fluorescent Mtb cells within 10 min of probe treatment. Thus, 3HC-3-Tre permits rapid visualization of mycobacteria that ultimately could empower improved Mtb detection at the point-of-care in low-resource settings.

摘要

迫切需要快速、廉价且操作简单的即时检测肺结核（TB）的诊断方法。在此，我们报告一种明亮的溶剂致变色染料海藻糖共轭物，它能在几分钟内特异性检测结核分枝杆菌（Mtb）。3-羟基色酮（3HC）染料以具有高荧光量子产率而闻名，其荧光强度会随环境极性的变化而发生偏移。我们合成了两种3HC-海藻糖共轭物类似物（3HC-2-Tre和3HC-3-Tre），并确定3HC-3-Tre具有用于检测Mtb的特别良好的性质。与我们之前关于染料4,4'-二甲基氨基萘二甲酰亚胺（DMN-Tre）的报告相比，用3HC-3-Tre标记的分枝杆菌细胞荧光强度增加了10倍。令人兴奋的是，在探针处理后10分钟内我们就检测到了荧光Mtb细胞。因此，3HC-3-Tre能快速实现分枝杆菌的可视化，最终可助力在资源匮乏地区的即时检测点更好地检测Mtb。