Department of Temporomandibular Disorders, Poznan University of Medical Sciences, Poznan, Poland.
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland.
BMC Oral Health. 2021 Oct 5;21(1):496. doi: 10.1186/s12903-021-01844-1.
Chronic stress is one of the leading predisposing factors in bruxism aetiology, but the influence of genetic factors is also suggested. We aimed to study whether sequence variants in genes involved in stress regulation pathways: NTRK2 and BDNF, may be associated with awake bruxism susceptibility, clinical presentation, and patients' perceived stress level.
The study group included 104 patients with probable awake bruxism and 191 population controls. Patients underwent dental examination concerning the symptoms of bruxism and psychological testing. Genotyping was performed using HRMA and sequencing. Statistical analyses were conducted, and P values below 0.05 were considered statistically significant.
We observed a positive correlation of measured stress level and pathological teeth attrition in the anterior segment (r = 0.45, P < 0.001), enamel attritions (r = 0.44, P < 0.001), tongue impressions (r = 0.50, P < 0.001) and posterior teeth attrition (r = 0.27, P = 0.005). Moreover, the c.196A variant (p.66Met) of the BDNF gene and c.1397-31392G allele of the NTRK2 gene were present with elevated frequency, comparing to controls.
This study hence the thesis that perceived stress level is a substantial contributing factor to awake bruxism occurrence and its clinical manifestations. Moreover, sequence variants in genes related to stress coping may be correlated with awake bruxism's susceptibility via elevated perceived stress level.
慢性应激是磨牙症发病的主要诱发因素之一，但遗传因素的影响也被认为是导致磨牙症发病的原因之一。我们旨在研究参与应激调节途径的基因（NTRK2 和 BDNF）中的序列变异是否与觉醒性磨牙症易感性、临床特征和患者感知的应激水平相关。
研究组包括 104 例可能患有觉醒性磨牙症的患者和 191 名对照人群。患者接受了有关磨牙症症状的牙科检查和心理测试。使用 HRMA 和测序进行基因分型。进行了统计分析，P 值<0.05 被认为具有统计学意义。
我们观察到测量的应激水平与前牙段病理性牙齿磨损（r=0.45，P<0.001）、牙釉质磨损（r=0.44，P<0.001）、舌印记（r=0.50，P<0.001）和后牙磨损（r=0.27，P=0.005）之间存在正相关。此外，BDNF 基因的 c.196A 变异（p.66Met）和 NTRK2 基因的 c.1397-31392G 等位基因的出现频率高于对照组。
因此，本研究认为感知的应激水平是觉醒性磨牙症发生及其临床表现的重要影响因素。此外，与应激应对相关的基因中的序列变异可能通过升高的感知应激水平与觉醒性磨牙症的易感性相关。
