分离并描述了牙龈炎患者中发现的新型 sp. nov.。

Isolation and description of sp. nov., a novel species detected in a gingivitis patient.

机构信息

Aix-Marseille Université, Faculté des Sciences Médicales et Paramédicales, Ecole de Médecine Dentaire, 27 Boulevard Jean Moulin, 13385 Marseille, France.

Assistance Publique-Hôpitaux de Marseille (AP-HM), Hôpital Timone, Service de Parodontologie, 264, Rue Saint Pierre, 13385 Marseille, France.

出版信息

Int J Syst Evol Microbiol. 2021 Oct;71(10). doi: 10.1099/ijsem.0.005040.

DOI:10.1099/ijsem.0.005040

PMID:34612809

Abstract

A Gram-stain-negative bacterium, designated strain Marseille-Q3039, was isolated from subgingival dental plaque of a woman with gingivitis in Marseille, France. Strain Marseille-Q3039 was found to be an anaerobic, motile and spore-forming crescent-shaped bacterium that grew at 25-41.5 °C (optimum, 37 °C), pH 5.5-8.5 (optimum, pH 7.5) and salinity of 5.0 g l NaCl. The results of 16S rRNA gene sequence analysis revealed that strain Marseille-Q3039 was closely related to ATCC 43532 (98.42 % similarity), ATCC 43527 (97.25 %) and DSM 2778 (97.19 %). The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness between strain Q3039 and its closest phylogenetic neighbours were respectively 84.57 and 28.2 % for ATCC 43532 and 83.93 and 27.2 % for DSM 2778. The major fatty acids were identified as C (27.7 %), C (24.4 %) and specific C 3-OH (12.3 %). Genome sequencing revealed a genome size of 2 351 779 bp and a G+C content of 57.2 mol%. On the basis of the results from phenotypic, chemotaxonomic, genomic and phylogenetic analyses and data, we concluded that strain Marseille-Q3039 represents a novel species of the genus , for which the name sp. nov. is proposed (=CSUR Q3039=CECT 30128).

摘要

一株革兰氏阴性细菌，被命名为马赛 Q3039 菌株，是从法国马赛一位患有牙龈炎的女性的龈下牙菌斑中分离出来的。马赛 Q3039 菌株被发现是一种厌氧、能动且能形成芽孢的新月形细菌，能在 25-41.5°C（最佳温度 37°C）、pH5.5-8.5（最佳 pH 值为 7.5）和 5.0g l NaCl 的盐度下生长。16S rRNA 基因序列分析的结果表明，菌株马赛 Q3039 与 ATCC 43532（98.42%相似性）、ATCC 43527（97.25%相似性）和 DSM 2778（97.19%相似性）最为密切相关。菌株 Q3039 与其最接近的系统发育邻居之间的直系同源平均核苷酸同一性和数字 DNA-DNA 杂交相关性分别为 84.57%和 28.2%，与 ATCC 43532 为 83.93%和 27.2%，与 DSM 2778 为 83.93%和 27.2%。主要脂肪酸为 C（27.7%）、C（24.4%）和特定 C3-OH（12.3%）。基因组测序显示基因组大小为 2351779bp，G+C 含量为 57.2mol%。基于表型、化学分类、基因组和系统发育分析以及数据的结果，我们得出结论，菌株马赛 Q3039 代表了一个新的属种，提议使用新的种名 sp. nov.（=CSUR Q3039=CECT 30128）。