sp. nov., an obligately anaerobic and volatile fatty acid producing bacterium from ruminal fluid of domestic goat ( L.).

A novel Gram-stain-negative, motile, obligately anaerobic bacterium strain mPRGC8 was isolated from the ruminal fluid of a domestic goat ( L.) in Nakhon Pathom province, Thailand. The strain grew at 20-45 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.5) and 3 % (w/v) NaCl. It produced acetate, propionate, valerate, caproate and heptanoate from glucose. The 16S rRNA gene sequence analysis indicated that strain mPRGC8 belonged to the genus and was closely related to subsp. DSM 2150 (98.0 %) and subsp. JCM 6582 (97.9 %). The DNA G+C content was 53.0 mol %. Strain mPRGC8 showed average nucleotide identity, digital DNA-DNA hybridization and average animo acid identity values with JCM 34373, subsp. JCM 6582 and subsp. DSM 2150 ranging from 84.9 to 86.0 %, 21.3 to 21.8 % and 73.8 to 76.1 %, respectively. The predominant cellular fatty acids were C 9 and C 9. Phosphatidylethanolamine, three unidentified aminophospholipids, two unidentified ninhydrin positive glycolipids, an unidentified phospholipid and an unidentified lipid were detected as polar lipids. The genomic and phenotypic characteristics of strain mPRGC8 strongly support its classification as representative of new species of the genus for which the name sp. nov. is proposed. The type strain is mPRGC8 (=JCM 33725=KCTC 25178).