Department of Animal Husbandry, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Int J Syst Evol Microbiol. 2023 Jun;73(6). doi: 10.1099/ijsem.0.005936.
A novel Gram-stain-negative, motile, obligately anaerobic bacterium strain mPRGC8 was isolated from the ruminal fluid of a domestic goat ( L.) in Nakhon Pathom province, Thailand. The strain grew at 20-45 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.5) and 3 % (w/v) NaCl. It produced acetate, propionate, valerate, caproate and heptanoate from glucose. The 16S rRNA gene sequence analysis indicated that strain mPRGC8 belonged to the genus and was closely related to subsp. DSM 2150 (98.0 %) and subsp. JCM 6582 (97.9 %). The DNA G+C content was 53.0 mol %. Strain mPRGC8 showed average nucleotide identity, digital DNA-DNA hybridization and average animo acid identity values with JCM 34373, subsp. JCM 6582 and subsp. DSM 2150 ranging from 84.9 to 86.0 %, 21.3 to 21.8 % and 73.8 to 76.1 %, respectively. The predominant cellular fatty acids were C 9 and C 9. Phosphatidylethanolamine, three unidentified aminophospholipids, two unidentified ninhydrin positive glycolipids, an unidentified phospholipid and an unidentified lipid were detected as polar lipids. The genomic and phenotypic characteristics of strain mPRGC8 strongly support its classification as representative of new species of the genus for which the name sp. nov. is proposed. The type strain is mPRGC8 (=JCM 33725=KCTC 25178).
一株新型革兰氏阴性、运动性、严格厌氧菌 mPRGC8 菌株从泰国那空巴统府的一只家养山羊的瘤胃液中分离得到。该菌株在 20-45 °C（最适温度为 37 °C）、pH 6.0-9.0（最适 pH 为 7.5）和 3%（w/v）NaCl 条件下生长。它可以利用葡萄糖产生乙酸盐、丙酸盐、戊酸盐、己酸盐和庚酸盐。16S rRNA 基因序列分析表明，菌株 mPRGC8 属于 属，与 subsp. DSM 2150（98.0%）和 subsp. JCM 6582（97.9%）最为密切相关。该菌株的 DNA G+C 含量为 53.0mol%。mPRGC8 菌株与 JCM 34373、DSM 2150 亚种、JCM 6582 亚种的平均核苷酸同一性、数字 DNA-DNA 杂交和平均氨基酸同一性值分别为 84.9-86.0%、21.3-21.8%和 73.8-76.1%。主要的细胞脂肪酸为 C9 和 C9。该菌含有磷脂酰乙醇胺、三种未鉴定的氨基磷脂、两种未鉴定的硝酮阳性糖脂、一种未鉴定的磷脂和一种未鉴定的脂类，作为极性脂类。mPRGC8 菌株的基因组和表型特征强烈支持其被归类为 属的新种代表，因此提议将其命名为 sp. nov。其模式菌株为 mPRGC8（=JCM 33725=KCTC 25178）。
