Okamoto Kazuhisa, Kodama Masaaki, Mizukami Kazuhiro, Okimoto Tadayoshi, Abe Hisanori, Ogawa Ryo, Fukuda Kensuke, Matsunari Osamu, Hirashita Yuka, Wada Yasuhiro, Fukuda Masahide, Murakami Kazunari
Department of Gastroenterology, Faculty of Medicine, Oita University, 1-1 Idaigaoka, Hasama-machi, Yufu, Oita 879-5593, Japan.
Faculty of Welfare and Health Science, Oita University, 700 Dannoharu, Oita 870-1192, Japan.
J Clin Biochem Nutr. 2021 Sep;69(2):216-221. doi: 10.3164/jcbn.20-179. Epub 2021 Mar 25.
In this study, the level of cell damage were analyzed immuno-histochemically to clarify the association between nodular gastritis and undifferentiated gastric cancer. Thirty patients of nodular gastritis were enrolled as the nodular gastritis group. Thirty patients of non-nodular gastritis were enrolled as the control group. They were evaluated according to the updated Sydney system and used for immunohistochemical staining (p53, Ki-67, E-cadherin, and 8-OHdG). The scores based on the updated Sydney system were significantly higher in the nodular group than in the non-nodular group for histologically assessed inflammation and activity in the gastric corpus (1.91 ± 0.77 vs 1.58 ± 0.60, = 0.049, 0.83 ± 0.81 vs 0.44 ± 0.64, = 0.032). On immunostaining, the detection of E-cadherin was lower in the nodular group for both the antrum (1.0 ± 0.62 vs 1.47 ± 0.85, = 0.047) and the corpus (1.16 ± 0.81 vs 1.48 ± 0.71, = 0.043) and the p53 labeling index of the gastric corpus was higher in the nodular group than in the non-nodular group (3.06 ± 1.94 vs 2.03 ± 1.99, = 0.015). Nodular gastritis showed significant severe inflammation and immunohistochemical cell damage compared with non-nodular gastritis. These findings may play an important role in the oncogenesis of undifferentiated gastric cancer in nodular gastritis.
在本研究中，采用免疫组织化学方法分析细胞损伤水平，以阐明结节性胃炎与未分化型胃癌之间的关联。纳入30例结节性胃炎患者作为结节性胃炎组。纳入30例非结节性胃炎患者作为对照组。根据更新后的悉尼系统对他们进行评估，并用于免疫组织化学染色（p53、Ki-67、E-钙黏蛋白和8-羟基脱氧鸟苷）。在胃体部，根据更新后的悉尼系统评估的组织学炎症和活动评分，结节组显著高于非结节组（1.91±0.77 vs 1.58±0.60，P=0.049；0.83±0.81 vs 0.44±0.64，P=0.032）。免疫染色显示，结节组胃窦部（1.0±0.62 vs 1.47±0.85，P=0.047）和胃体部（1.16±0.81 vs 1.48±0.71，P=0.043）的E-钙黏蛋白检测率均低于非结节组，且结节组胃体部的p53标记指数高于非结节组（3.06±1.94 vs 2.03±1.99，P=0.015）。与非结节性胃炎相比，结节性胃炎表现出明显更严重的炎症和免疫组织化学细胞损伤。这些发现可能在结节性胃炎未分化型胃癌的发生中起重要作用。
