Khoza Siyabonga P, Ford Sarah, Buthelezi Ernest P, Tanyanyiwa Donald M
University of the Witwatersrand, Johannesburg, South Africa.
National Health Laboratory Service, Department of Chemical Pathology, Johannesburg, South Africa.
J Med Biochem. 2021 Sep 3;40(4):358-366. doi: 10.5937/jomb0-27216.
Barricor Lithium heparin plasma tubes are new blood tubes that have been introduced to overcome the effects of gel in serum separator tubes (SST) and the shortcomings of standard Lithium heparin plasma. We aimed to evaluate Barricor tubes as an alternative to serum separator tubes and compare the stability between the tubes.
Forty-four paired samples were collected using both Barricor and SST. We compared five analytes at baseline (<6 h) and after every 24 h using the PassingBablok and Bland-Altman plots. Aspartate aminotransferase (AST), potassium (K), phosphate (PO4) , lactate dehydrogenase (LDH), and creatinine were analysed in both tubes. We calculated the percentage difference for each analyte between the baseline and time intervals to assess analyte stability. The percentage difference was compared to the desirable specification for bias and reference change value (RCV).
All analytes were comparable at baseline. Statistical differences (p<0.001) became evident after 24 h. PO4, K, and creatinine had a mean difference that exceeded the desirable specification for bias (-9.59%, - 9.35%, and -4.59%, respectively). Potassium was stable up to 24 h in both tubes. LDH showed better stability in SST (144 h vs 96 h). PO4 concentrations were more stable in both tubes with the SST (96 h vs 72 h). Creatinine and AST had the longest stability in both tubes compared to other analytes (144 h).
Data demonstrated variability and similarities in analyte concentrations and stability, respectively, in both tubes.
Barricor锂肝素血浆管是新推出的采血管，旨在克服血清分离管（SST）中凝胶的影响以及标准锂肝素血浆的缺点。我们旨在评估Barricor管作为血清分离管的替代品，并比较两种管之间的稳定性。
使用Barricor管和SST管收集44对配对样本。我们使用PassingBablok和Bland-Altman图在基线（<6小时）以及之后每24小时比较五种分析物。在两种管中分析天冬氨酸转氨酶（AST）、钾（K）、磷酸盐（PO4）、乳酸脱氢酶（LDH）和肌酐。我们计算每种分析物在基线和各时间间隔之间的百分比差异，以评估分析物的稳定性。将百分比差异与偏差和参考变化值（RCV）的理想规格进行比较。
所有分析物在基线时具有可比性。24小时后出现统计学差异（p<0.001）。PO4、K和肌酐的平均差异超过了偏差的理想规格（分别为-9.59%、-9.35%和-4.59%）。钾在两种管中直至24小时都是稳定的。LDH在SST管中显示出更好的稳定性（144小时对96小时）。PO4浓度在SST管中两种管内都更稳定（96小时对72小时）。与其他分析物相比，肌酐和AST在两种管中的稳定性最长（144小时）。
数据表明两种管中分析物浓度分别存在变异性和相似性，以及稳定性方面的情况。
